Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit | EAAT3 elisa kit

Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit

Cat. Num. AAA452267

• Gene Names: SLC1A1; DCBXA; EAAC1; EAAT3; SCZD18
• Reactivity: Human
• Synonyms: Excitatory Amino Acid Transporter 3 (EAAT3); Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit; ITG-A11; EAAT3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of EAAT3. No significant cross-reactivity or interference between EAAT3 and analogues was observed.
• Sequence Length: 524

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.117ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EAAT3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EAAT3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EAAT3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EAAT3 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EAAT3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EAAT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EAAT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EAAT3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 66773030
• NCBI GeneID: 6505
• NCBI Accession #: NP_004161.4
• NCBI GenBank Nucleotide #: NM_004170.5
• UniProt Accession #: P43005; O75587; Q5VZ24; Q8N199; Q9UEW2
• Molecular Weight: 57,100 Da
• NCBI Official Full Name: excitatory amino acid transporter 3
• NCBI Official Synonym Full Names: solute carrier family 1 member 1
• NCBI Official Symbol: SLC1A1
• NCBI Official Synonym Symbols: DCBXA; EAAC1; EAAT3; SCZD18
• NCBI Protein Information: excitatory amino acid transporter 3
• UniProt Protein Name: Excitatory amino acid transporter 3
• UniProt Gene Name: SLC1A1
• UniProt Synonym Gene Names: EAAC1; EAAT3

NCBI Description

This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are thought to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect. [provided by RefSeq, Mar 2010]

Uniprot Description

Transports L-glutamate, L- and D-aspartate and L-cystein (PubMed:21123949). Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5 ().