Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit | EAAT3 elisa kit

Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit

Cat. Num. AAA2023644

• Gene Names: SLC1A1; DCBXA; EAAC1; EAAT3; SCZD18
• Reactivity: Human
• Synonyms: Excitatory Amino Acid Transporter 3 (EAAT3); Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit; SLC1A1; EAAC1; Solute Carrier 1 Member 1; Sodium-dependent glutamate/aspartate transporter 3; Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; EAAT3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Excitatory Amino Acid Transporter 3 (EAAT3).; No significant cross-reactivity or interference between Excitatory Amino Acid Transporter 3 (EAAT3) and analogues was observed.
• Sequence Length: 524

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.117ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 3 (EAAT3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 3 (EAAT3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EAAT3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excitatory Amino Acid Transporter 3 (EAAT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Excitatory Amino Acid Transporter 3 (EAAT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excitatory Amino Acid Transporter 3 (EAAT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Excitatory Amino Acid Transporter 3 (EAAT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for EAAT3 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 66773030
• NCBI GeneID: 6505
• NCBI Accession #: NP_004161.4
• NCBI GenBank Nucleotide #: NM_004170.5
• UniProt Accession #: P43005; O75587; Q5VZ24; Q8N199; Q9UEW2
• Molecular Weight: 57,100 Da
• NCBI Official Full Name: excitatory amino acid transporter 3
• NCBI Official Synonym Full Names: solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
• NCBI Official Symbol: SLC1A1
• NCBI Official Synonym Symbols: DCBXA; EAAC1; EAAT3; SCZD18
• NCBI Protein Information: excitatory amino acid transporter 3
• UniProt Protein Name: Excitatory amino acid transporter 3
• UniProt Gene Name: SLC1A1
• UniProt Synonym Gene Names: EAAC1; EAAT3
• UniProt Entry Name: EAA3_HUMAN

NCBI Description

This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are thought to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect. [provided by RefSeq, Mar 2010]

Uniprot Description

SLC1A1: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily.

Protein type: Transporter, SLC family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: membrane; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: glutamate binding; cysteine transmembrane transporter activity; glutamate:sodium symporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity

Biological Process: L-glutamate import; synaptic transmission; cysteine transport; ion transport; transmembrane transport; protein homooligomerization

Disease: Schizophrenia 18