Rat Dual Oxidase 2 (DUOX2) ELISA Kit | DUOX2 elisa kit

Rat Dual Oxidase 2 (DUOX2) ELISA Kit

Cat. Num. AAA452991

• Gene Names: Duox2; Thox2
• Reactivity: Rat
• Synonyms: Dual Oxidase 2 (DUOX2); Rat Dual Oxidase 2 (DUOX2) ELISA Kit; LNOX2; NOXEF2; THOX2; Thyroid oxidase 2; NADH/NADPH thyroid oxidase p138-tox; Dual Oxidase-Like Domains 2; Nicotinamide Adenine Dinucleotide Phosphate Oxidase; DUOX2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of DUOX2. No significant cross-reactivity or interference between DUOX2 and analogues was observed.
• Sequence Length: 456

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DUOX2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DUOX2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DUOX2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DUOX2 in rat serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DUOX2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DUOX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DUOX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DUOX2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13162322
• NCBI GeneID: 79107
• NCBI Accession #: NP_077055.1
• NCBI GenBank Nucleotide #: NM_024141.1
• UniProt Accession #: Q9ES45; Q811Y4
• Molecular Weight: 52,576 Da
• NCBI Official Full Name: dual oxidase 2
• NCBI Official Synonym Full Names: dual oxidase 2
• NCBI Official Symbol: Duox2
• NCBI Official Synonym Symbols: Thox2
• NCBI Protein Information: dual oxidase 2
• UniProt Protein Name: Dual oxidase 2
• Protein Family: Dual oxidase
• UniProt Gene Name: Duox2
• UniProt Synonym Gene Names: Lnox2; Thox2

NCBI Description

a thyroid NADPH oxidase flavoprotein [RGD, Feb 2006]

Uniprot Description

Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.