Human INT8 (Integrator complex subunit 8) ELISA Kit | INT8 elisa kit

Human INT8 (Integrator complex subunit 8) ELISA Kit

Cat. Num. AAA7606708

• Gene Names: INTS8; INT8; C8orf52
• Reactivity: Human
• Synonyms: INT8 (Integrator complex subunit 8); Human INT8 (Integrator complex subunit 8) ELISA Kit; DBI1; DDX26; DDX26A; Protein deleted in cancer 1; DICE1; INT8 elisa kit

• Reactivity: Human
• Sequence Length: 995

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INT8 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- INT8 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-INT8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the INT8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of INT8 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 70608109
• NCBI GeneID: 55656
• NCBI Accession #: NP_060334.2
• NCBI GenBank Nucleotide #: NM_017864.3
• UniProt Accession #: Q75QN2; Q5RKZ3; Q6P1R5; Q7Z314; Q9NVS6; Q9NWY7; B2RN92
• Molecular Weight: 111,116 Da
• NCBI Official Full Name: integrator complex subunit 8
• NCBI Official Synonym Full Names: integrator complex subunit 8
• NCBI Official Symbol: INTS8
• NCBI Official Synonym Symbols: INT8; C8orf52
• NCBI Protein Information: integrator complex subunit 8
• UniProt Protein Name: Integrator complex subunit 8
• UniProt Gene Name: INTS8
• UniProt Synonym Gene Names: C8orf52; Int8

NCBI Description

This gene encodes a subunit of the Integrator complex which is involved in the cleavage of small nuclear RNAs U1 and U2 within the nucleus. The encoded protein associates with RNA polymerase II and is recruited to the U1 and U2 small nuclear RNA genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Uniprot Description

INTS8: Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: integrator complex; nucleoplasm

Molecular Function: protein binding

Biological Process: snRNA processing; snRNA transcription from RNA polymerase II promoter