Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dual oxidase 2 (DUOX2) Standard Curve.)
Human Dual Oxidase 2 (DUOX2) ELISA Kit | DUOX2 elisa kit
Human Dual Oxidase 2 (DUOX2) ELISA Kit
Gene Names
DUOX2; TDH6; LNOX2; THOX2; NOXEF2; P138-TOX
Reactivity
Human
Synonyms
Dual Oxidase 2 (DUOX2); Human Dual Oxidase 2 (DUOX2) ELISA Kit; DUOX2; LNOX2; NOXEF2; P138-TOX; TDH6; THOX2; NADH/NADPH thyroid oxidase p138-tox; NADPH oxidase/peroxidase DUOX2; NADPH thyroid oxidase 2; dual oxidase-like domains 2; flavoprotein NADPH oxidase; nicotinamide a; DUOX2 elisa kit
Reactivity
Human
Specificity
Human Dual oxidase 2 (DUOX2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DUOX2. No significant cross-reactivity or interference between Human DUOX2 and analogues was observed.
Sequence Length
1548
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dual oxidase 2 (DUOX2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dual oxidase 2 (DUOX2) Standard Curve.)
Related Product Information for DUOX2 elisa kit
Description: This Human Dual oxidase 2 (DUOX2) ELISA Kit employs a two-site sandwich ELISA to quantitate DUOX2 in samples. An antibody specific for DUOX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DUOX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DUOX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DUOX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Dual oxidase is an enzyme that was first identified in the mammalian thyroid gland. In humans, two isoforms are found; hDUOX1 and hDUOX2 (this enzyme). The protein location is not exclusive to thyroid tissue; hDUOX1 is prominent in airway epithelial cells and hDUOX2 in the salivary glands and gastrointestinal tract.It has been suggested that DUOX2 is the isoform to generate H2O2 utilized by thyroid peroxidase (TPO) for the biosynthesis of thyroid hormones, supported by the discovery of congenital hypothyroidism resultant from an inactivating mutation in the DUOX2 gene. The family currently has seven members including NOX1, NOX2 (formerly known as gp91phox), NOX3, NOX4, NOX5, DUOX1 and DUOX2.This protein is known as a dual oxidase because it has both a peroxidase homology domain and a gp91phox domain.
Background: Dual oxidase is an enzyme that was first identified in the mammalian thyroid gland. In humans, two isoforms are found; hDUOX1 and hDUOX2 (this enzyme). The protein location is not exclusive to thyroid tissue; hDUOX1 is prominent in airway epithelial cells and hDUOX2 in the salivary glands and gastrointestinal tract.It has been suggested that DUOX2 is the isoform to generate H2O2 utilized by thyroid peroxidase (TPO) for the biosynthesis of thyroid hormones, supported by the discovery of congenital hypothyroidism resultant from an inactivating mutation in the DUOX2 gene. The family currently has seven members including NOX1, NOX2 (formerly known as gp91phox), NOX3, NOX4, NOX5, DUOX1 and DUOX2.This protein is known as a dual oxidase because it has both a peroxidase homology domain and a gp91phox domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dual oxidase 2
NCBI Official Synonym Full Names
dual oxidase 2
NCBI Official Symbol
DUOX2
NCBI Official Synonym Symbols
TDH6; LNOX2; THOX2; NOXEF2; P138-TOX
NCBI Protein Information
dual oxidase 2
UniProt Protein Name
Dual oxidase 2
Protein Family
UniProt Gene Name
DUOX2
UniProt Synonym Gene Names
LNOX2; THOX2
NCBI Description
The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX1. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. [provided by RefSeq, Jul 2008]
Uniprot Description
Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.
Research Articles on DUOX2
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Product Notes
The Human DUOX2 duox2 (Catalog #AAA9715633) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715633 ELISA Kit recognizes Human DUOX2. It is sometimes possible for the material contained within the vial of "Dual Oxidase 2 (DUOX2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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