Rat DNA Methyltransferase 1 (DNMT1) ELISA Kit | DNMT1 elisa kit

Rat DNA Methyltransferase 1 (DNMT1) ELISA Kit

Cat. Num. AAA2706622

• Gene Names: Dnmt1; Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
• Reactivity: Rat
• Synonyms: DNA Methyltransferase 1 (DNMT1); Rat DNA Methyltransferase 1 (DNMT1) ELISA Kit; CXXC9; DNMT; MCMT; Cytosine-5 DNA Methyltransferase 1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNMT1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of DNA Methyltransferase 1 (DNMT1).; No significant cross-reactivity or interference between DNA Methyltransferase 1 (DNMT1) and analogues was observed.
• Sequence Length: 1,502

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DNA Methyltransferase 1 (DNMT1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DNA Methyltransferase 1 (DNMT1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DNMT1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Methyltransferase 1 (DNMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Methyltransferase 1 (DNMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Methyltransferase 1 (DNMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Methyltransferase 1 (DNMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1765919
• NCBI GeneID: 13433
• UniProt Accession #: P13864; P97413; Q80ZU3; Q9CSC6; Q9QXX6
• Molecular Weight: 169,996 Da
• NCBI Official Full Name: DNA methyltransferase 1
• NCBI Official Synonym Full Names: DNA methyltransferase (cytosine-5) 1
• NCBI Official Symbol: Dnmt1
• NCBI Official Synonym Symbols: Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 1
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 1
• UniProt Gene Name: Dnmt1
• UniProt Synonym Gene Names: Dnmt; Met1; Uim; Dnmt1; Met-1; DNA MTase MmuI; M.MmuI

NCBI Description

This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (). Promotes tumor growth ().