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DNMT1 Protein Recombinant Protein | DNMT1 recombinant protein
Recombinant Mouse DNMT1 Protein (His Tag)
Gene Names
Dnmt1; Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
Purity
>80% as determined by reducing SDS-PAGE
Synonyms
DNMT1 Protein; Recombinant Mouse DNMT1 Protein (His Tag); DNA (cytosine-5)-methyltransferase 1; Dnmt1; Met-1; DNA methyltransferase MmuI; DNA MTase MmuI; M.MmuI; MCMT; Dnmt; Met1; Uim; DNMT1 recombinant protein
Host
E Coli
Purity/Purification
>80% as determined by reducing SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4
Sequence
Leu1396-Pro1583
Species
Mouse
Tag
N-His
Protein Construction
A DNA sequence encoding the Mouse DNMT1 (P13864-1)(Leu1396-Pro1583) was expressed with a polyhistidine tag at the N-terminus.
Preparation and Storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
This product is provided as lyophilized powder which is shipped with ice packs.
This product is provided as lyophilized powder which is shipped with ice packs.
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SDS-Page
Related Product Information for DNMT1 recombinant protein
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
169,996 Da
NCBI Official Full Name
DNA methyltransferase 1
NCBI Official Synonym Full Names
DNA methyltransferase (cytosine-5) 1
NCBI Official Symbol
Dnmt1
NCBI Official Synonym Symbols
Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA MTase MmuI; DNA methyltransferase MmuI
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 1
UniProt Gene Name
Dnmt1
UniProt Synonym Gene Names
Dnmt; Met1; Uim; Dnmt1; Met-1; DNA MTase MmuI; M.MmuI
UniProt Entry Name
DNMT1_MOUSE
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Product Notes
The DNMT1 dnmt1 (Catalog #AAA2569309) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Leu1396-Pr o1583. It is sometimes possible for the material contained within the vial of "DNMT1 Protein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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