Typical Testing Data/Standard Curve
Rat DNA Methyltransferase 1 ELISA Kit | DNMT1 elisa kit
Rat DNA Methyltransferase 1 (DNMT1) ELISA Kit
No significant cross-reactivity or interference between DNA Methyltransferase 1 (DNMT1) and analogues was observed.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.
Protein type: Methyltransferase, DNA; Cell development/differentiation; Amino Acid Metabolism - cysteine and methionine; Transcription regulation; Methyltransferase; EC 2.1.1.37
Cellular Component: cell soma; centric heterochromatin; cytoplasm; heterochromatin; nucleus; protein complex; replication fork
Molecular Function: catalytic activity; chromatin binding; DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; DNA-methyltransferase activity; double-stranded DNA binding; estrogen receptor binding; histone deacetylase binding; metal ion binding; methyl-CpG binding; methyltransferase activity; protein binding; protein domain specific binding; RNA binding; transcription factor binding; transferase activity; unmethylated CpG binding; zinc ion binding
Biological Process: chromatin modification; cytosine methylation within a CG sequence; DNA methylation; DNA methylation on cytosine; gene silencing; maintenance of DNA methylation; metabolic process; methylation; negative regulation of histone H3-K9 methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of histone H3-K4 methylation; regulation of cell proliferation; regulation of gene expression; regulation of transcription, DNA-dependent; S-adenosylhomocysteine metabolic process; S-adenosylmethioninamine metabolic process; S-adenosylmethionine metabolic process; transcription, DNA-dependent
Research Articles on DNMT1
Similar Products
Product Notes
The Rat DNMT1 dnmt1 (Catalog #AAA2031849) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2031849 ELISA Kit recognizes Rat DNMT1. It is sometimes possible for the material contained within the vial of "DNA Methyltransferase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
