Rat Deiodinase, Iodothyronine, Type III (DIO3) ELISA Kit | DIO3 elisa kit

Rat Deiodinase, Iodothyronine, Type III (DIO3) ELISA Kit

Cat. Num. AAA2024064

• Gene Names: DIO3; D3; 5DIII; TXDI3; DIOIII
• Reactivity: Rat
• Synonyms: Deiodinase; Iodothyronine; Type III (DIO3); Rat Deiodinase; Type III (DIO3) ELISA Kit; ITDI3; TXDI3; Type-III 5'-deiodinase; DIO3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Deiodinase, Iodothyronine, Type III (DIO3).; No significant cross-reactivity or interference between Deiodinase, Iodothyronine, Type III (DIO3) and analogues was observed.
• Sequence Length: 304

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.117ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Deiodinase, Iodothyronine, Type III (DIO3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Deiodinase, Iodothyronine, Type III (DIO3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DIO3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deiodinase, Iodothyronine, Type III (DIO3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deiodinase, Iodothyronine, Type III (DIO3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deiodinase, Iodothyronine, Type III (DIO3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deiodinase, Iodothyronine, Type III (DIO3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DIO3 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 56103188
• NCBI GeneID: 1735
• UniProt Accession #: P55073; Q8WVN5; G3XAM0
• Molecular Weight: 33,947 Da
• NCBI Official Full Name: Deiodinase, iodothyronine, type III
• NCBI Official Synonym Full Names: deiodinase, iodothyronine, type III
• NCBI Official Symbol: DIO3
• NCBI Official Synonym Symbols: D3; 5DIII; TXDI3; DIOIII
• NCBI Protein Information: type III iodothyronine deiodinase
• UniProt Protein Name: Type III iodothyronine deiodinase
• Protein Family: Thyroxine 5-deiodinase
• UniProt Gene Name: DIO3
• UniProt Synonym Gene Names: ITDI3; TXDI3
• UniProt Entry Name: IOD3_HUMAN

NCBI Description

The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

Uniprot Description

DIO3: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. Belongs to the iodothyronine deiodinase family.

Protein type: Oxidoreductase; Membrane protein, integral; EC 1.97.1.11

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: integral to membrane; plasma membrane; endosome membrane

Molecular Function: thyroxine 5'-deiodinase activity; thyroxine 5-deiodinase activity

Biological Process: thyroid hormone catabolic process; thyroid hormone generation; positive regulation of multicellular organism growth; hormone biosynthetic process