Human Very low-density lipoprotein receptor ELISA Kit | VLDLR elisa kit

Human Very low-density lipoprotein receptor ELISA Kit

Cat. Num. AAA763633

• Reactivity: Human
• Synonyms: Very low-density lipoprotein receptor; Human Very low-density lipoprotein receptor ELISA Kit; VLDLR (Very low-density lipoprotein receptor)/CHRMQ1/VLDLRCH/CARMQ1/very low density lipoprotein receptor/VLDL receptor/VLDL-R; VLDLR elisa kit

• Reactivity: Human
• Sequence Length: 873

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 1.875ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for VLDLR elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-VLDLR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-VLDLR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VLDLR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VLDLR can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 688371
• Molecular Weight: 93,383 Da
• NCBI Official Full Name: very low density lipoprotein receptor
• UniProt Protein Name: Very low-density lipoprotein receptor
• Protein Family: Very low-density lipoprotein receptor
• UniProt Gene Name: VLDLR
• UniProt Synonym Gene Names: VLDL receptor; VLDL-R
• UniProt Entry Name: VLDLR_HUMAN

Uniprot Description

VLDLR: Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1); also known as dysequilibrium syndrome (DES) or non- progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: apical part of cell; cell surface; coated pit; integral to membrane; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; receptor complex

Molecular Function: apolipoprotein binding; calcium ion binding; calcium-dependent protein binding; glycoprotein binding; low-density lipoprotein receptor activity; protein binding; very-low-density lipoprotein binding; very-low-density lipoprotein receptor activity

Biological Process: cellular response to glucose starvation; cellular response to insulin stimulus; cerebral cortex development; cholesterol metabolic process; dendrite morphogenesis; heart development; lipid transport; memory; negative regulation of transcription from RNA polymerase II promoter; nervous system development; positive regulation of protein kinase activity; receptor-mediated endocytosis; response to drug; response to nutrient; signal transduction; ventral spinal cord development

Disease: Cerebellar Ataxia, Mental Retardation, And Dysequilibrium Syndrome 1