Human Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1) ELISA Kit | AMIGO1 elisa kit

Human Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1) ELISA Kit

Cat. Num. AAA2707276

• Gene Names: Amigo1; ali2; Amigo
• Reactivity: Human
• Synonyms: Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1); Human Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1) ELISA Kit; ALI2; Alivin-2; Amphoterin-Induced Gene And Open Reading Frame; Amphoterin-induced protein 1; AMIGO1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1).; No significant cross-reactivity or interference between Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1) and analogues was observed.
• Sequence Length: 309

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.064ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AMIGO1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 52430466
• NCBI GeneID: 229715
• UniProt Accession #: Q80ZD8; Q69ZQ0; Q8R5D4; Q921U9; A2AE40
• Molecular Weight: 35,661 Da
• NCBI Official Full Name: Adhesion molecule with Ig like domain 1
• NCBI Official Synonym Full Names: adhesion molecule with Ig like domain 1
• NCBI Official Symbol: Amigo1
• NCBI Official Synonym Symbols: ali2; Amigo
• NCBI Protein Information: amphoterin-induced protein 1
• UniProt Protein Name: Amphoterin-induced protein 1
• Protein Family: Amphoterin-induced protein
• UniProt Gene Name: Amigo1

Uniprot Description

Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain (). Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:22056818).