Rat DAF ELISA Kit | DAF elisa kit
Rat DAF (Decay Accelerating Factor) ELISA Kit
Reactivity
Rat
Synonyms
DAF; Rat DAF (Decay Accelerating Factor) ELISA Kit; DAF elisa kit
Reactivity
Rat
Specificity
This kit recognizes natural and recombinant Rat DAF. No significant cross-reactivity or interference between Rat DAF and analogues was observed.
Sequence Length
10
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
Min: 18.75pg/mL; Max: 2000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DAF elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat DAF concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DAF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DAF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of DAF. You can calculate the concentration of DAF in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DAF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DAF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of DAF. You can calculate the concentration of DAF in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
1,063 Da
NCBI Official Full Name
decay-accelerating factor, partial
UniProt Protein Name
Decay-accelerating factor
Protein Family
UniProt Gene Name
DAF
UniProt Entry Name
V9GZY1_HUMAN
NCBI Description
This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]
