WB (Western Blot)
(Western blot analysis of anti-MEK1 Antibody (S218/222) in A2058, A375 cell line lysates (35ug/lane). MEK1 (arrow) was detected using the purified Pab.)
Rabbit MEK1 Polyclonal Antibody | anti-MAP2K1 antibody
MEK1 Antibody (S218/222)
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human (Predicted Reactivity: Drosophila, Hamster, Mouse, Rabbit, Rat, Xenopus, Chicken)
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MEK1, Antibody; MEK1 Antibody (S218/222); Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK1; PRKMK1; anti-MAP2K1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Drosophila, Hamster, Mouse, Rabbit, Rat, Xenopus, Chicken)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MEK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-234 amino acids from human MEK1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.37 (varies by lot)
Sequence Positions
199-234
Sequence Length
367
Applicable Applications for anti-MAP2K1 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of anti-MEK1 Antibody (S218/222) in A2058, A375 cell line lysates (35ug/lane). MEK1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-MEK1 Antibody (S218/222) in A2058, A375 cell line lysates (35ug/lane). MEK1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of lysate from rat C6 cell line, using MEK1 Antibody (S218/222). AAA289071 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
WB (Western Blot)
(Western blot analysis of lysate from rat C6 cell line, using MEK1 Antibody (S218/222). AAA289071 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Related Product Information for anti-MAP2K1 antibody
MAP2K1 is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Product Categories/Family for anti-MAP2K1 antibody
References
Zheng, B., et al., Blood 102(3):1019-1027 (2003).
Li, S.P., et al., Cancer Res. 63(13):3473-3477 (2003).
Zhu, X., et al., J. Neurochem. 86(1):136-142 (2003).
Fringer, J., et al., J. Biol. Chem. 278(23):20612-20617 (2003).
Witowsky, J.A., et al., J. Biol. Chem. 278(3):1403-1406 (2003).
Li, S.P., et al., Cancer Res. 63(13):3473-3477 (2003).
Zhu, X., et al., J. Neurochem. 86(1):136-142 (2003).
Fringer, J., et al., J. Biol. Chem. 278(23):20612-20617 (2003).
Witowsky, J.A., et al., J. Biol. Chem. 278(3):1403-1406 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43439
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 1
NCBI Official Symbol
MAP2K1
NCBI Official Synonym Symbols
CFC3; MEK1; MKK1; MAPKK1; PRKMK1
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 1
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 1
UniProt Gene Name
MAP2K1
UniProt Synonym Gene Names
MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1
UniProt Entry Name
MP2K1_HUMAN
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Product Notes
The MAP2K1 map2k1 (Catalog #AAA289071) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 199-234. The MEK1 Antibody (S218/222) reacts with Human (Predicted Reactivity: Drosophila, Hamster, Mouse, Rabbit, Rat, Xenopus, Chicken) and may cross-react with other species as described in the data sheet. AAA Biotech's MEK1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MAP2K1 map2k1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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