Rat D Site Of Albumin Promoter Binding Protein (DBP) ELISA Kit | DBP elisa kit

Rat D Site Of Albumin Promoter Binding Protein (DBP) ELISA Kit

Cat. Num. AAA456265

• Reactivity: Rat
• Synonyms: D Site Of Albumin Promoter Binding Protein (DBP); Rat D Site Of Albumin Promoter Binding Protein (DBP) ELISA Kit; Clipin-A; Coro1; Coronin-1A; Coronin-like protein A; TACO; Tryptophan aspartate-containing coat protein; DBP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of DBP. No significant cross-reactivity or interference between DBP and analogues was observed.
• Sequence Length: 325

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.105ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DBP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DBP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DBP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DBP in rat tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DBP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DBP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7549756
• NCBI GeneID: 24309
• NCBI Accession #: NP_036675.1
• NCBI GenBank Nucleotide #: NM_012543.3
• UniProt Accession #: P16443
• Molecular Weight: 34,436 Da
• NCBI Official Full Name: D site-binding protein isoform 1
• NCBI Official Synonym Full Names: D-box binding PAR bZIP transcription factor
• NCBI Official Symbol: Dbp
• NCBI Protein Information: D site-binding protein
• UniProt Protein Name: D site-binding protein
• Protein Family: D site-binding protein
• UniProt Gene Name: Dbp

NCBI Description

The protein encoded by this gene is a member of the Par bZIP transcription factor family and binds to specific sequences in the promoters of several genes, such as albumin, Cyp2a4, and Cyp2a5. The encoded protein can bind DNA as a homo- or heterodimer and is involved in the regulation of some circadian rhythym genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation ().