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Typical Testing Data/Standard Curve (for reference only) ()

Rat Dihydrotestosterone (DHT) ELISA Kit | DHT elisa kit

Rat Dihydrotestosterone (DHT) ELISA Kit

Reactivity
Rat
Synonyms
Dihydrotestosterone (DHT); Rat Dihydrotestosterone (DHT) ELISA Kit; DHT elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
No cross-reaction with other factors.
Sequence Length
381
Samples
Rat serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
2000 pg/mL-31.2 pg/mL
Sensitivity
Up to 12 pg/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

()

Typical Testing Data/Standard Curve (for reference only) ()
Related Product Information for DHT elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat DHT monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
43,009 Da
NCBI Official Full Name
differentially repressed by testosterone and dihydrotestosterone
UniProt Protein Name
Protein NDRG1
UniProt Gene Name
Ndrg1
UniProt Synonym Gene Names
Ndr1; Ndrl; Tdd5; Protein Ndr1
UniProt Entry Name
NDRG1_MOUSE

Uniprot Description

NDRG1: a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D).

Protein type: Cell development/differentiation; Vesicle

Cellular Component: microtubule cytoskeleton; microtubule; centrosome; cell-cell adherens junction; cytoskeleton; membrane; perinuclear region of cytoplasm; recycling endosome membrane; cytoplasm; plasma membrane; nucleus; myelin sheath

Molecular Function: gamma-tubulin binding; cadherin binding; microtubule binding; Rab GTPase binding

Biological Process: myelin maintenance in the peripheral nervous system; mast cell activation

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Product Notes

The Rat DHT ndrg1 (Catalog #AAA265478) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA265478 ELISA Kit recognizes Rat DHT. It is sometimes possible for the material contained within the vial of "Dihydrotestosterone (DHT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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