Typical Testing Data/Standard Curve (for reference only)
Rat D Site Of Albumin Promoter Binding Protein (DBP) ELISA Kit | DBP elisa kit
Rat D Site Of Albumin Promoter Binding Protein (DBP) ELISA Kit
Gene Names
DBP; DABP
Reactivity
Rat
Synonyms
D Site Of Albumin Promoter Binding Protein (DBP); Rat D Site Of Albumin Promoter Binding Protein (DBP) ELISA Kit; DABP; TaxREB302; Albumin D box-binding protein; Albumin D-element-binding protein; Tax-responsive enhancer element-binding protein 302; DBP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of D Site Of Albumin Promoter Binding Protein (DBP).
No significant cross-reactivity or interference between D Site Of Albumin Promoter Binding Protein (DBP) and analogues was observed.
No significant cross-reactivity or interference between D Site Of Albumin Promoter Binding Protein (DBP) and analogues was observed.
Sequence Length
325
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates and Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.105ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level D Site Of Albumin Promoter Binding Protein (DBP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level D Site Of Albumin Promoter Binding Protein (DBP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DBP elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D Site Of Albumin Promoter Binding Protein (DBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D Site Of Albumin Promoter Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D Site Of Albumin Promoter Binding Protein (DBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D Site Of Albumin Promoter Binding Protein (DBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for DBP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,349 Da
NCBI Official Full Name
D site of albumin promoter (albumin D-box) binding protein variant, partial
NCBI Official Synonym Full Names
D site of albumin promoter (albumin D-box) binding protein
NCBI Official Symbol
DBP
NCBI Official Synonym Symbols
DABP
NCBI Protein Information
D site-binding protein
UniProt Protein Name
D site-binding protein
Protein Family
UniProt Gene Name
DBP
UniProt Synonym Gene Names
TaxREB302
UniProt Entry Name
DBP_HUMAN
NCBI Description
The protein encoded by this gene is a member of the PAR bZIP transcription factor family and binds to specific sequences in the promoters of several genes, such as albumin, CYP2A4, and CYP2A5. The encoded protein can bind DNA as a homo- or heterodimer and is involved in the regulation of some circadian rhythm genes. [provided by RefSeq, Jul 2014]
Uniprot Description
DBP: This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation. Belongs to the bZIP family. PAR subfamily.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleus
Biological Process: regulation of transcription from RNA polymerase II promoter; circadian rhythm; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; liver development
Research Articles on DBP
Similar Products
Product Notes
The Rat DBP dbp (Catalog #AAA2024598) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024598 ELISA Kit recognizes Rat DBP. It is sometimes possible for the material contained within the vial of "D Site Of Albumin Promoter Binding Protein (DBP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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