Rat D (3) dopamine receptor (DRD3) ELISA Kit | DRD3 elisa kit

Rat D (3) dopamine receptor (DRD3) ELISA Kit

Cat. Num. AAA2800152

• Reactivity: Rat
• Synonyms: D (3) dopamine receptor (DRD3); Rat D (3) dopamine receptor (DRD3) ELISA Kit; D3DR; ETM1; FET1; MGC149204; MGC149205; essential tremor 1; DRD3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat DRD3. No significant cross-reactivity or interference between Rat DRD3 and analogues was observed.
• Sequence Length: 446

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DRD3 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate DRD3 in samples. An antibody specific for DRD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DRD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DRD3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DRD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: D3 dopamine receptor inhibits adenylyl cyclase through inhibitory G-proteins. This receptor is expressed in phylogenetically older regions of the brain, suggesting that this receptor plays a role in cognitive and emotional functions. It is a target for drugs which treat schizophrenia, drug addiction, and Parkinson's disease. Alternative splicing of this gene results in multiple transcript variants that would encode different isoforms, although some variants may be subject to nonsense-mediated decay. Numerous non-selective prescription drugs bind to the D3 receptor, notably including some of the newer dopamine agonists used for Parkinson's disease such as pramipexole and ropinirole, but these also bind to D2 and lack the strong receptor type and subtype selectivity that some of the following research compounds afford.

NCBI and Uniprot Product Information

• NCBI GI #: 8393271
• NCBI GeneID: 29238
• NCBI Accession #: NP_058836.1
• NCBI GenBank Nucleotide #: NM_017140.2
• UniProt Accession #: P19020; P70647
• Molecular Weight: 47,633 Da
• NCBI Official Full Name: D(3) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D3
• NCBI Official Symbol: Drd3
• NCBI Protein Information: D(3) dopamine receptor
• UniProt Protein Name: D(3) dopamine receptor
• Protein Family: D(3) dopamine receptor
• UniProt Gene Name: Drd3

NCBI Description

may be involved in facilitating the effects of antipsychotic drugs and drug treatments for Parkinson's disease [RGD, Feb 2006]

Uniprot Description

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation ().