Typical Testing Data/Standard Curve (for reference only)
Mouse DRD3 ELISA Kit | DRD3 elisa kit
Mouse DRD3 (Dopamine Receptor D3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DRD3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DRD3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DRD3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of DRD3. You can calculate the concentration of DRD3 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the D3 subtype of the five (D1-D5) dopamine receptors. The activity of the D3 subtype receptor is mediated by G proteins which inhibit adenylyl cyclase. This receptor is localized to the limbic areas of the brain, which are associated with cognitive, emotional, and endocrine functions. Genetic variation in this gene may be associated with susceptibility to hereditary essential tremor 1. Alternative splicing of this gene results in transcript variants encoding different isoforms, although some variants may be subject to nonsense-mediated decay (NMD). [provided by RefSeq, Jul 2008]
Uniprot Description
DRD3: Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. Genetic variation in DRD3 is associated with essential tremor hereditary type 1 (ETM1). ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR
Chromosomal Location of Human Ortholog: 3q13.3
Cellular Component: cell projection; apical part of cell; endocytic vesicle; integral to plasma membrane; plasma membrane
Molecular Function: dopamine D2 receptor-like receptor activity; protein domain specific binding; protein binding; dopamine binding; drug binding; D1 dopamine receptor binding
Biological Process: synaptic transmission, dopaminergic; regulation of lipid metabolic process; prepulse inhibition; musculoskeletal movement, spinal reflex action; response to morphine; locomotory behavior; negative regulation of transcription from RNA polymerase II promoter; dopamine metabolic process; regulation of dopamine secretion; dopamine receptor, adenylate cyclase inhibiting pathway; learning and/or memory; behavioral response to cocaine; positive regulation of cell proliferation; renin-angiotensin regulation of blood volume; visual learning; negative regulation of blood pressure; circadian regulation of gene expression; negative regulation of sodium:hydrogen antiporter activity; dopamine receptor, adenylate cyclase activating pathway; response to drug; positive regulation of cytokinesis; positive regulation of dopamine receptor signaling pathway; negative regulation of protein secretion; negative regulation of protein kinase B signaling cascade; positive regulation of mitosis; regulation of cAMP metabolic process; response to amphetamine; negative regulation of adenylate cyclase activity; regulation of dopamine uptake; regulation of multicellular organism growth; learning; social behavior; arachidonic acid secretion; response to cocaine; regulation of circadian sleep/wake cycle, sleep; cellular calcium ion homeostasis; negative regulation of oligodendrocyte differentiation; G-protein coupled receptor protein signaling pathway; acid secretion; response to ethanol; negative regulation of dopamine receptor signaling pathway; G-protein coupled receptor internalization; positive regulation of transcription from RNA polymerase II promoter
Disease: Schizophrenia; Tremor, Hereditary Essential, 1
Research Articles on DRD3
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Product Notes
The Mouse DRD3 drd3 (Catalog #AAA2511605) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2511605 ELISA Kit recognizes Mouse DRD3. It is sometimes possible for the material contained within the vial of "DRD3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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