Rat Cytochrome P450 Reductase (CPR) ELISA Kit | CPR elisa kit

Rat Cytochrome P450 Reductase (CPR) ELISA Kit

Cat. Num. AAA2705039

• Gene Names: POR; CPR; CYPOR; P450R
• Reactivity: Rat
• Synonyms: Cytochrome P450 Reductase (CPR); Rat Cytochrome P450 Reductase (CPR) ELISA Kit; POR; CYPOR; P450R; P450(Cytochrome)Oxidoreductase; NADPH--cytochrome P450 reductase; CPR elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 Reductase (CPR).; No significant cross-reactivity or interference between Cytochrome P450 Reductase (CPR) and analogues was observed.
• Sequence Length: 677

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.107ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 Reductase (CPR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 Reductase (CPR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CPR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 Reductase (CPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 Reductase (CPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 Reductase (CPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 Reductase (CPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 247307
• NCBI GeneID: 5447
• UniProt Accession #: P16435; Q16455; Q197M5; Q8N181; Q9H3M8; Q9UDT3
• Molecular Weight: 76,690 Da
• NCBI Official Full Name: cytochrome P450 reductase, partial
• NCBI Official Synonym Full Names: cytochrome p450 oxidoreductase
• NCBI Official Symbol: POR
• NCBI Official Synonym Symbols: CPR; CYPOR; P450R
• NCBI Protein Information: NADPH--cytochrome P450 reductase
• UniProt Protein Name: NADPH--cytochrome P450 reductase
• Protein Family: Probable oxidosqualene cyclase
• UniProt Gene Name: POR
• UniProt Synonym Gene Names: CPR; P450R

NCBI Description

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.