Human Cytochrome P450 Reductase ELISA Kit | CPR elisa kit

Human Cytochrome P450 Reductase ELISA Kit

Cat. Num. AAA763754

• Gene Names: POR; CPR; CYPOR; P450R
• Reactivity: Human
• Synonyms: Cytochrome P450 Reductase; Human Cytochrome P450 Reductase ELISA Kit; CPR/CYPOR/Cytochrome P450 Reductase/P450R/EC 1.6.2.4/NADPH--cytochrome P450 reductase/NADPH-dependent cytochrome P450 reductase/P450 (cytochrome) oxidoreductase; CPR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CPR. No significant cross-reactivity or interference between CPR and analogues was observed.
• Sequence Length: 676

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CPR elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CPR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CPR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CPR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CPR can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 247307
• NCBI GeneID: 5447
• Molecular Weight: 76,690 Da
• NCBI Official Full Name: cytochrome P450 reductase, partial
• NCBI Official Synonym Full Names: P450 (cytochrome) oxidoreductase
• NCBI Official Symbol: POR
• NCBI Official Synonym Symbols: CPR; CYPOR; P450R
• NCBI Protein Information: NADPH--cytochrome P450 reductase
• UniProt Protein Name: NADPH--cytochrome P450 reductase
• Protein Family: Probable oxidosqualene cyclase
• UniProt Gene Name: POR
• UniProt Synonym Gene Names: CPR; P450R
• UniProt Entry Name: NCPR_HUMAN

NCBI Description

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

POR: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Defects in POR are the cause of Antley-Bixler syndrome with genital anomalies and disordered steroidogenesis (ABS1). A disease characterized by the association of Antley-Bixler syndrome with steroidogenesis defects and abnormal genitalia. Antley-Bixler syndrome is characterized by craniosynostosis, radiohumeral synostosis present from the perinatal period, midface hypoplasia, choanal stenosis or atresia, femoral bowing and multiple joint contractures. Defects in POR are the cause of disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD). A disorder resulting in a rare variant of congenital adrenal hyperplasia, with apparent combined P450C17 and P450C21 deficiency and accumulation of steroid metabolites. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.

Protein type: EC 1.6.2.4; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q11.2

Cellular Component: endoplasmic reticulum membrane; integral to membrane; intracellular membrane-bound organelle; membrane; mitochondrion

Molecular Function: [methionine synthase] reductase activity; cytochrome-b5 reductase activity; electron carrier activity; enzyme binding; FAD binding; FMN binding; hydrolase activity; iron ion binding; iron-cytochrome-c reductase activity; NADP binding; NADPH-hemoprotein reductase activity; nitric oxide dioxygenase activity; protein binding

Biological Process: carnitine metabolic process; fatty acid oxidation; flavonoid metabolic process; internal peptidyl-lysine acetylation; negative regulation of caspase activity; negative regulation of lipase activity; nitrate catabolic process; nitric oxide catabolic process; positive regulation of cholesterol biosynthetic process; positive regulation of chondrocyte differentiation; positive regulation of monooxygenase activity; positive regulation of smoothened signaling pathway; response to drug; response to nutrient

Disease: Antley-bixler Syndrome With Genital Anomalies And Disordered Steroidogenesis; Antley-bixler Syndrome Without Genital Anomalies Or Disordered Steroidogenesis; Disordered Steroidogenesis Due To Cytochrome P450 Oxidoreductase Deficiency