Rabbit anti-Human, Mouse YY1 Polyclonal Antibody | anti-YY1 antibody

YY1, ID (Transcriptional Repressor Protein YY1, Yin And Yang 1, YY-1, Delta Transcription Factor, NF-E1, INO80 Complex Subunit S, INO80S) (Biotin)

Cat. Num. AAA6503325

• Gene Names: YY1; DELTA; NF-E1; UCRBP; INO80S; YIN-YANG-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: YY1; Polyclonal Antibody; ID (Transcriptional Repressor Protein YY1; Yin And Yang 1; YY-1; Delta Transcription Factor; NF-E1; INO80 Complex Subunit S; INO80S) (Biotin); anti-YY1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human YY1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-YY1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human YY1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-YY1 Ctr Pab in HL60 cell lysate (lane 1) and in mouse kidney tissue lysate (lane 2). YY1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-YY1 antibody

Antibodies; Transcription Factors

References

1. Sucharov, C.C., et al., J. Biol. Chem. 278(33):31233-31239 (2003). 2. Kurisaki, K., et al., Mol. Cell. Biol. 23(13):4494-4510 (2003). 3. Huang, N.E., et al., Biochem. Biophys. Res. Commun. 306(1):267-275 (2003). 4. Moriuchi, M., et al., J. Biol. Chem. 278(15):13003-13007 (2003). 5. Hiromura, M., et al., J. Biol. Chem. 278(16):14046-14052 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 459683878
• NCBI GeneID: 7528
• NCBI Accession #: NP_003394.1
• NCBI GenBank Nucleotide #: NM_003403
• UniProt Accession #: P25490; Q14935
• Molecular Weight: 44,713 Da
• NCBI Official Full Name: Homo sapiens YY1 transcription factor (YY1), mRNA
• NCBI Official Synonym Full Names: YY1 transcription factor
• NCBI Official Symbol: YY1
• NCBI Official Synonym Symbols: DELTA; NF-E1; UCRBP; INO80S; YIN-YANG-1
• NCBI Protein Information: transcriptional repressor protein YY1; INO80 complex subunit S; YY-1; Yin and Yang 1 protein; delta transcription factor
• UniProt Protein Name: Transcriptional repressor protein YY1
• Protein Family: YY1-associated factor
• UniProt Gene Name: YY1
• UniProt Synonym Gene Names: INO80S; YY-1
• UniProt Entry Name: TYY1_HUMAN

NCBI Description

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq, Jul 2008]