Human Cytochrome P450 Reductase (CPR) ELISA Kit | CPR elisa kit

Human Cytochrome P450 Reductase (CPR) ELISA Kit

Cat. Num. AAA2023374

• Gene Names: POR; CPR; CYPOR; P450R
• Reactivity: Human
• Synonyms: Cytochrome P450 Reductase (CPR); Human Cytochrome P450 Reductase (CPR) ELISA Kit; POR; CYPOR; P450R; P450(Cytochrome)Oxidoreductase; NADPH--cytochrome P450 reductase; CPR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CPN1. No significant cross-reactivity or interference between CPN1 and analogues was observed.
• Sequence Length: 677

• Samples: Human Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: 6.7pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CPN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CPN1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CPR elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CPN1 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CPN1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CPN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CPN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CPN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CPR elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 247307
• NCBI GeneID: 5447
• UniProt Accession #: P16435; Q16455; Q197M5; Q8N181; Q9H3M8; Q9UDT3
• Molecular Weight: 76,690 Da
• NCBI Official Full Name: cytochrome P450 reductase, partial
• NCBI Official Synonym Full Names: P450 (cytochrome) oxidoreductase
• NCBI Official Symbol: POR
• NCBI Official Synonym Symbols: CPR; CYPOR; P450R
• NCBI Protein Information: NADPH--cytochrome P450 reductase
• UniProt Protein Name: NADPH--cytochrome P450 reductase
• Protein Family: Probable oxidosqualene cyclase
• UniProt Gene Name: POR
• UniProt Synonym Gene Names: CYPOR; CPR; P450R
• UniProt Entry Name: NCPR_HUMAN

NCBI Description

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

POR: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Defects in POR are the cause of Antley-Bixler syndrome with genital anomalies and disordered steroidogenesis (ABS1). A disease characterized by the association of Antley-Bixler syndrome with steroidogenesis defects and abnormal genitalia. Antley-Bixler syndrome is characterized by craniosynostosis, radiohumeral synostosis present from the perinatal period, midface hypoplasia, choanal stenosis or atresia, femoral bowing and multiple joint contractures. Defects in POR are the cause of disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD). A disorder resulting in a rare variant of congenital adrenal hyperplasia, with apparent combined P450C17 and P450C21 deficiency and accumulation of steroid metabolites. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.

Protein type: EC 1.6.2.4; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q11.2

Cellular Component: endoplasmic reticulum membrane; mitochondrion; membrane; intracellular membrane-bound organelle

Molecular Function: protein binding; enzyme binding; FAD binding; electron carrier activity; NADPH-hemoprotein reductase activity; hydrolase activity; FMN binding; nitric oxide dioxygenase activity; cytochrome-b5 reductase activity; iron ion binding; NADP binding; iron-cytochrome-c reductase activity

Biological Process: response to drug; nitric oxide catabolic process; nitrate catabolic process; positive regulation of chondrocyte differentiation; internal peptidyl-lysine acetylation; flavonoid metabolic process; positive regulation of monooxygenase activity; positive regulation of cholesterol biosynthetic process; positive regulation of smoothened signaling pathway; carnitine metabolic process; fatty acid oxidation; negative regulation of caspase activity; negative regulation of lipase activity; response to nutrient

Disease: Disordered Steroidogenesis Due To Cytochrome P450 Oxidoreductase Deficiency; Antley-bixler Syndrome With Genital Anomalies And Disordered Steroidogenesis; Antley-bixler Syndrome Without Genital Anomalies Or Disordered Steroidogenesis