Rat Glutathione S-transferase alpha-4 ELISA Kit | Gsta4 elisa kit

Rat Glutathione S-transferase alpha-4 ELISA Kit

Cat. Num. AAA288053

• Reactivity: Rat
• Synonyms: Glutathione S-transferase alpha-4; Rat Glutathione S-transferase alpha-4 ELISA Kit; Gsta4 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural Rat Glutathione S-transferase alpha-4. No significant cross-reactivity or interference was observed.
• Sequence Length: 222

• Assay Type: Competitive
• Detection Range: 0.78-50 ng/mL
• Sensitivity: 0.442 ng/mL
• Intra-Assay CV: <=7.3%
• Inter-Assay CV: <=10.8%
• Recovery: 99%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Gsta4 elisa kit

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S-transferase alpha-4, During the reaction, Glutathione S-transferase alpha-4 in the sample or standard competes with a fixed amount of biotin-labeled Glutathione S-transferase alpha-4 for sites on a pre-coated Monoclonal antibody specific to Glutathione S-transferase alpha-4. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Glutathione S-transferase alpha-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 157820217
• NCBI GeneID: 300850
• NCBI Accession #: NP_001100310.1
• NCBI GenBank Nucleotide #: NM_001106840.1
• UniProt Accession #: P14942
• Molecular Weight: 25,510 Da
• NCBI Official Full Name: glutathione S-transferase alpha-4
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 4
• NCBI Official Symbol: Gsta4
• NCBI Protein Information: glutathione S-transferase alpha-4
• UniProt Protein Name: Glutathione S-transferase alpha-4
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: Gsta4
• UniProt Synonym Gene Names: GST Yk
• UniProt Entry Name: GSTA4_RAT

Uniprot Description

GSTA4: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 2.5.1.18

Cellular Component: cytoplasm; mitochondrion

Molecular Function: drug binding; glutathione binding; glutathione transferase activity; protein homodimerization activity

Biological Process: glutathione metabolic process; response to herbicide; response to nicotine; response to zinc ion; xenobiotic metabolic process