uBD recombinant protein

uBD, 1-165aa, Human, His tag, E Coli

Cat. Num. AAA204599

• Gene Names: UBD; FAT10; UBD-3; GABBR1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: uBD; 1-165aa; Human; His tag; E Coli; ubiquitin D; Diubiquitin; FAT10; GABBR1; uBD-3; uBD recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAPNASC LCVHVRSEEW DLMTFDANPY DSVKKIKEHV RSKTKVPVQD QVLLLGSKIL KPRRSLSSYG IDKEKTIHLT LKVVKPSDEE LPLFLVESGD EAKRHLLQVR RSSSVAQVKA MIETKTGIIP ETQIVTCNGK RLEDGKMMAD YGIRKGNLLF LACYCIGG
• Sequence Length: 165

• Applicable Applications for uBD recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for uBD recombinant protein

uBD, also known as ubiquitin D, is an ubiquitin-like modifier (uBL) of the ubiquitin protein family. uBD is an ubiquitin-like protein that can form covalent conjugates, thereby targeting proteins for degradation by the 26S proteasome. uBD has putative roles in regulation of cell cycle, innate immunity, and apoptosis. uBD is a TNF-alpha-inducible ubiquitin-like protein with a putative role in immune response. Recombinant human uBD protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for uBD recombinant protein

Ubiquitination

References

Hipp M.S., et al. (2005) Mol. Cell. Biol. 25:3483-3491; Ross M.J., et al. (2006) J. Am. Soc. Nephrol. 17:996-1004

NCBI and Uniprot Product Information

• NCBI GI #: 222352096
• NCBI GeneID: 10537
• NCBI Accession #: NP_006389
• NCBI GenBank Nucleotide #: NM_006398.3
• UniProt Accession #: O15205; Q5STL2; Q5SUK2; Q96EC7; B0UZT6
• Molecular Weight: 20.9 kDa (188aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ubiquitin D
• NCBI Official Synonym Full Names: ubiquitin D
• NCBI Official Symbol: UBD
• NCBI Official Synonym Symbols: FAT10; UBD-3; GABBR1
• NCBI Protein Information: ubiquitin D
• UniProt Protein Name: Ubiquitin D
• Protein Family: Ubiquitin
• UniProt Gene Name: UBD
• UniProt Synonym Gene Names: FAT10

NCBI Description

This gene encodes a protein which contains two ubiquitin-like domains and appears to have similar function to ubiquitin. Through covalent attachment, the encoded protein targets other proteins for 26S proteasome degradation. This protein has been implicated to function in many cellular processes, including caspase-dependent apoptosis, formation of aggresomes, mitotic regulation, and dendritic cell maturation. Upregulation of this gene may promote inflammation in chronic kidney disease and has been observed in many cancer types. [provided by RefSeq, Aug 2017]

Uniprot Description

Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1-dependent manner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN).