Typical Testing Data/Standard Curve (for reference only)
Rat CNTF ELISA Kit | CNTF elisa kit
Rat CNTF ELISA Kit
Reactivity
Rat
Applications
ELISA
Synonyms
CNTF; Rat CNTF ELISA Kit; Ciliary neurotrophic factor; CNTF elisa kit
Reactivity
Rat
Specificity
The Mouse RSPO3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse RSPO3 proteins within the range of 15.6 pg/ml-1000 pg/ml.
Sequence Length
200
Applicable Applications for CNTF elisa kit
Sandwich ELISA (SE)
Samples
Cell Culture Supernates, Serum, Plasma
Sensitivity
10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CNTF elisa kit
Principle of the Assay: The MyBioSource's Mouse RSPO3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse RSPO3 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse RSPO3 coated on a 96-well plate. Standards and samples are pipetted into the wells and RSPO3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse RSPO3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RSPO3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: R-spondin-3 is a protein that in humans is encoded by the RSPO3 gene. This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,854 Da
NCBI Official Full Name
ciliary neurotrophic factor
NCBI Official Synonym Full Names
ciliary neurotrophic factor
NCBI Official Symbol
Cntf
NCBI Protein Information
ciliary neurotrophic factor; ciliary neurotropic factor
UniProt Protein Name
Ciliary neurotrophic factor
Protein Family
UniProt Gene Name
Cntf
UniProt Synonym Gene Names
CNTF
UniProt Entry Name
CNTF_RAT
NCBI Description
a cytokine involved in neuronal degeneration and adipocyte gene expression [RGD, Feb 2006]
Uniprot Description
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Research Articles on CNTF
Similar Products
Product Notes
The Rat CNTF cntf (Catalog #AAA824896) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA824896 ELISA Kit recognizes Rat CNTF. AAA Biotech's CNTF can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (SE). Researchers should empirically determine the suitability of the CNTF cntf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CNTF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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