Rat CCL5 ELISA Kit | CCL5 elisa kit

Rat CCL5 ELISA Kit

Cat. Num. AAA824475

• Gene Names: Ccl5; Scya5; Rantes
• Reactivity: Rat
• Applications: ELISA
• Synonyms: CCL5; Rat CCL5 ELISA Kit; D17S136E; SCYA5; C-C motif chemokine 5; EoCP; Eosinophil chemotactic cytokine; SIS-delta; Small-inducible cytokine A5; T cell-specific protein P228; TCP228; T-cell-specific protein RANTES; CCL5 elisa kit

• Reactivity: Rat
• Specificity: The Rat CCL5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat CCL5 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 92

• Applicable Applications for CCL5 elisa kit: Sandwich ELISA (SE)
• Samples: Cell culture supernatants, serum, plasma
• Sensitivity: < 1 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL5 elisa kit

Principle of the Assay: The MyBioSource' Rat CCL5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CCL5 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat CCL5 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

NCBI and Uniprot Product Information

• NCBI GI #: 1711393
• NCBI GeneID: 81780
• UniProt Accession #: P50231
• Molecular Weight: 10,170 Da
• NCBI Official Full Name: C-C motif chemokine 5
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: Ccl5
• NCBI Official Synonym Symbols: Scya5; Rantes
• NCBI Protein Information: C-C motif chemokine 5; SIS-delta; small inducible cytokine A5; small-inducible cytokine A5; T-cell-specific protein RANTES; regulated upon activation normal T-cell expressed and secreted
• UniProt Protein Name: C-C motif chemokine 5
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl5
• UniProt Synonym Gene Names: Scya5
• UniProt Entry Name: CCL5_RAT

NCBI Description

may play a role in cellular response to viral infection in testicular somatic cells [RGD, Feb 2006]

Uniprot Description

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.