Rabbit anti-Human PLCL1 Polyclonal Antibody | anti-PLCL1 antibody

PLCL1, NT (Phospholipase C-related But Catalytically Inactive Protein, PRIP, Phospholipase C-deleted In Lung Carcinoma, Inactive Phospholipase C-like Protein 1, PLC-L1) (PE)

Cat. Num. AAA6493288

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: PLCL1; Polyclonal Antibody; NT (Phospholipase C-related But Catalytically Inactive Protein; PRIP; Phospholipase C-deleted In Lung Carcinoma; Inactive Phospholipase C-like Protein 1; PLC-L1) (PE); anti-PLCL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLCL1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 997

• Applicable Applications for anti-PLCL1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PLCL1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PLCL1 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). PLCL1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(PLCL1 Antibody (N-term) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PLCL1 antibody

Antibodies; Enzymes; Phospholipase

References

1. Liu, Y.Z., et.al., PLoS ONE 3(9), E3160 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 167900450
• NCBI Accession #: NP_006217.2
• NCBI GenBank Nucleotide #: NP_006217.2
• UniProt Accession #: Q15111
• Molecular Weight: 113.0122
• NCBI Official Full Name: phospholipase C-like 1 isoform b
• UniProt Protein Name: Inactive phospholipase C-like protein 1
• Protein Family: Inactive phospholipase C-like protein
• UniProt Gene Name: PLCL1
• UniProt Synonym Gene Names: PLC-L1; PRIP
• UniProt Entry Name: PLCL1_HUMAN

Uniprot Description

PLCL1: a protein closely related to phospholipase C-d1 (PLC-d1) but catalytically inactive. First identified as a novel inositol 1,4,5-trisphosphate binding protein. Has a number of binding partners including protein phosphatase (PP1 and 2A), GABA(A) receptor associated protein, and the beta subunits of GABA(A) receptors, in addition to inositol 1,4,5-trisphosphate. Plays an important role in controlling the dynamics of GABA(A) receptor phosphorylation through PP1 binding and, therefore, the efficacy of synaptic inhibition mediated by these receptors.

Protein type: Phospholipase

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: cytoplasm; plasma membrane

Molecular Function: signal transducer activity; GABA receptor binding; phosphoinositide phospholipase C activity; phospholipase C activity

Biological Process: regulation of peptidyl-serine phosphorylation; lipid metabolic process; regulation of synaptic transmission, GABAergic; gamma-aminobutyric acid signaling pathway