Human CCAAT/Enhancer Binding Protein Alpha ELISA Kit | C/EBPalpha elisa kit

Human CCAAT/Enhancer Binding Protein Alpha ELISA Kit

Cat. Num. AAA764745

• Gene Names: CEBPA; CEBP; C/EBP-alpha
• Reactivity: Human
• Synonyms: CCAAT/Enhancer Binding Protein Alpha; Human CCAAT/Enhancer Binding Protein Alpha ELISA Kit; C/EBPalpha ; C/EBPalpha elisa kit

• Reactivity: Human
• Sequence Length: 357

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for C/EBPalpha elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human EBPalpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human EBPalpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EBPalpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human EBPalpha can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1877205
• NCBI GeneID: 1050
• UniProt Accession #: P49715; P78319; Q05CA4; A7LNP2
• Molecular Weight: 41,249 Da
• NCBI Official Full Name: CCAAT/enhancer binding protein alpha
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein alpha
• NCBI Official Symbol: CEBPA
• NCBI Official Synonym Symbols: CEBP; C/EBP-alpha
• NCBI Protein Information: CCAAT/enhancer-binding protein alpha
• UniProt Protein Name: CCAAT/enhancer-binding protein alpha
• UniProt Gene Name: CEBPA
• UniProt Synonym Gene Names: C/EBP alpha
• UniProt Entry Name: CEBPA_HUMAN

NCBI Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]

Uniprot Description

C/EBP-alpha: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-beta and CEBP-gamma. Binds to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: nucleolus; nucleus

Molecular Function: DNA binding; kinase binding; protein binding; protein homodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: brown fat cell differentiation; cell maturation; cholesterol metabolic process; cytokine and chemokine mediated signaling pathway; embryonic placenta development; fat cell differentiation; generation of precursor metabolites and energy; glucose homeostasis; granulocyte differentiation; inner ear development; lipid homeostasis; liver development; lung development; macrophage differentiation; mitochondrion organization and biogenesis; myeloid cell differentiation; negative regulation of cell proliferation; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; Notch signaling pathway; positive regulation of fat cell differentiation; positive regulation of osteoblast differentiation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase III promoter; transcription from RNA polymerase I promoter; transcription from RNA polymerase II promoter; transcription, DNA-dependent; urea cycle; viral reproduction; white fat cell differentiation

Disease: Leukemia, Acute Myeloid