Human CCAAT/Enhancer Binding Protein Alpha (CEBPa) ELISA Kit | CEBPa elisa kit

Human CCAAT/Enhancer Binding Protein Alpha (CEBPa) ELISA Kit

Cat. Num. AAA454275

• Gene Names: CEBPA; CEBP; C/EBP-alpha
• Reactivity: Human
• Synonyms: CCAAT/Enhancer Binding Protein Alpha (CEBPa); Human CCAAT/Enhancer Binding Protein Alpha (CEBPa) ELISA Kit; CEBP-A; CEBPa elisa kit

• Reactivity: Human
• Sequence Length: 239

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• Intra Assay: CV<10%
• Inter Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 551894998
• NCBI GeneID: 1050
• NCBI Accession #: NP_001272758.1
• NCBI GenBank Nucleotide #: NM_001285829.1
• UniProt Accession #: P49715; P78319; Q05CA4; A7LNP2
• Molecular Weight: 41,249 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein alpha isoform b
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein alpha
• NCBI Official Symbol: CEBPA
• NCBI Official Synonym Symbols: CEBP; C/EBP-alpha
• NCBI Protein Information: CCAAT/enhancer-binding protein alpha
• UniProt Protein Name: CCAAT/enhancer-binding protein alpha
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: CEBPA
• UniProt Synonym Gene Names: C/EBP alpha

NCBI Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]

Uniprot Description

Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes (PubMed:11242107). During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung (). Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (). To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Downregulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Proliferation arrest also depends on a functional binding to SWI/SNF complex (PubMed:14660596). In liver, regulates gluconeogenesis and lipogenesis through different mechanisms. To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC. To modulate lipogenesis, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2. In adipose tissue, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites ().