Human Calbindin ELISA Kit | CALB1 elisa kit

Human Calbindin ELISA Kit

Cat. Num. AAA760684

• Gene Names: CALB1; CALB; D-28K
• Reactivity: Human
• Synonyms: Calbindin; Human Calbindin ELISA Kit; CALB1 (Calbindin)/CAB27/Vitamin D-dependent calcium-binding protein; avian-type/Calbindin D28/D-28K; CALB1 elisa kit

• Reactivity: Human
• Sequence Length: 261

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 6.25-400pg/ml
• Sensitivity: 3.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CALB1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human CALB1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CALB1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CALB1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CALB1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4826655
• NCBI GeneID: 793
• NCBI Accession #: NP_004920.1
• NCBI GenBank Nucleotide #: NM_004929.3
• Molecular Weight: 23,609 Da
• NCBI Official Full Name: calbindin
• NCBI Official Synonym Full Names: calbindin 1
• NCBI Official Symbol: CALB1
• NCBI Official Synonym Symbols: CALB; D-28K
• NCBI Protein Information: calbindin
• UniProt Protein Name: Calbindin
• Protein Family: Calbindin
• UniProt Gene Name: CALB1
• UniProt Synonym Gene Names: CAB27
• UniProt Entry Name: CALB1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]

Uniprot Description

CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Interacts with RANBP9. Belongs to the calbindin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: axon; cell soma; cytosol; dendrite; nucleus; synapse; terminal button

Molecular Function: calcium ion binding; protein binding; vitamin D binding; zinc ion binding

Biological Process: cytosolic calcium ion homeostasis; locomotory behavior; long-term memory; regulation of synaptic plasticity; short-term memory