Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit | Bcl2L2 elisa kit

Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit

Cat. Num. AAA2706910

• Gene Names: BCL2; Bcl-2; PPP1R50
• Reactivity: Rat
• Synonyms: B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2); Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit; BCL-W; BCLW; PPP1R51; Apoptosis regulator Bcl-W; Bcl-2-like protein 2; Bcl2L2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2).; No significant cross-reactivity or interference between B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) and analogues was observed.
• Sequence Length: 205

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.067ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Bcl2L2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 20072668
• NCBI GeneID: 596
• Molecular Weight: 22,337 Da
• NCBI Official Full Name: B-cell CLL/lymphoma 2
• NCBI Official Synonym Full Names: BCL2, apoptosis regulator
• NCBI Official Symbol: BCL2
• NCBI Official Synonym Symbols: Bcl-2; PPP1R50
• NCBI Protein Information: apoptosis regulator Bcl-2
• UniProt Protein Name: Apoptosis regulator Bcl-2
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2

NCBI Description

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).