Mouse B-Cell CLL/Lymphoma 9 (Bcl9) ELISA Kit | Bcl9 elisa kit

Mouse B-Cell CLL/Lymphoma 9 (Bcl9) ELISA Kit

Cat. Num. AAA2024467

• Gene Names: BCL9; LGS
• Reactivity: Mouse
• Synonyms: B-Cell CLL/Lymphoma 9 (Bcl9); Mouse B-Cell CLL/Lymphoma 9 (Bcl9) ELISA Kit; LGS; Protein legless homolog; Bcl9 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of B-Cell CLL/Lymphoma 9 (Bcl9).; No significant cross-reactivity or interference between B-Cell CLL/Lymphoma 9 (Bcl9) and analogues was observed.
• Sequence Length: 1,426

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 78.12-5000pg/mL
• Sensitivity: < 27pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 9 (Bcl9) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 9 (Bcl9) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Bcl9 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 9 (Bcl9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 9 (Bcl9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 9 (Bcl9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell CLL/Lymphoma 9 (Bcl9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for Bcl9 elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 94574438
• NCBI GeneID: 607
• UniProt Accession #: O00512; Q5T489
• Molecular Weight: 149,290 Da
• NCBI Official Full Name: B-cell CLL/lymphoma 9
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 9
• NCBI Official Symbol: BCL9
• NCBI Official Synonym Symbols: LGS
• NCBI Protein Information: B-cell CLL/lymphoma 9 protein
• UniProt Protein Name: B-cell CLL/lymphoma 9 protein
• Protein Family: B-cell CLL/lymphoma 9 protein
• UniProt Gene Name: BCL9
• UniProt Synonym Gene Names: B-cell lymphoma 9 protein; Bcl-9
• UniProt Entry Name: BCL9_HUMAN

NCBI Description

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Bcl-9: Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity. A chromosomal aberration involving BCL9 is found in a patient with precusor B-cell acute lymphoblastic leukemia (ALL). Translocation t(1;14)(q21;q32). This translocation leaves the coding region intact, but may have pathogenic effects due to alterations in the expression level of BCL9. Several cases of translocations within the 3'-UTR of BCL9 have been found in B-cell malignancies. Belongs to the BCL9 family.

Protein type: Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; Golgi apparatus; cis-Golgi network

Molecular Function: protein binding; beta-catenin binding

Biological Process: regulation of transforming growth factor beta receptor signaling pathway; somatic stem cell maintenance; myotube differentiation involved in skeletal muscle regeneration; positive regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin