Rat Aspartate Aminotransferase (AST) ELISA Kit | AST elisa kit

Rat Aspartate Aminotransferase (AST) ELISA Kit

Cat. Num. AAA451210

• Gene Names: Got1; ASAT; cCAT; Aspat; Gaspat; cAspAT
• Reactivity: Rat
• Synonyms: Aspartate Aminotransferase (AST); Rat Aspartate Aminotransferase (AST) ELISA Kit; cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase; cytoplasmic; Transaminase A; Aspartate Transaminase 1; Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1; Soluble; AST elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of AST. No significant cross-reactivity or interference between AST and analogues was observed.
• Sequence Length: 413

• Samples: Rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: <0.117ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AST were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AST were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AST elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AST in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AST. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AST in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 401461792
• NCBI GeneID: 24401
• NCBI Accession #: NP_036703.2
• NCBI GenBank Nucleotide #: NM_012571.2
• UniProt Accession #: P13221; Q64570; Q6P721
• Molecular Weight: 46,429 Da
• NCBI Official Full Name: aspartate aminotransferase, cytoplasmic
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 1
• NCBI Official Symbol: Got1
• NCBI Official Synonym Symbols: ASAT; cCAT; Aspat; Gaspat; cAspAT
• NCBI Protein Information: aspartate aminotransferase, cytoplasmic
• UniProt Protein Name: Aspartate aminotransferase, cytoplasmic
• Protein Family: Allatostatin
• UniProt Gene Name: Got1
• UniProt Synonym Gene Names: cAspAT; cCAT

NCBI Description

cytosolic aspartate aminotransferase isozyme; enzyme activity in liver and kidney increase in response to glucocorticoids [RGD, Feb 2006]

Uniprot Description

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.