Rat Apolipoprotein A5 (APOA5) ELISA Kit | APOA5 elisa kit

Rat Apolipoprotein A5 (APOA5) ELISA Kit

Cat. Num. AAA454437

• Gene Names: Apoa5; apo-AV; apoA-V
• Reactivity: Rat
• Synonyms: Apolipoprotein A5 (APOA5); Rat Apolipoprotein A5 (APOA5) ELISA Kit; APOA5 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of APOA5. No significant cross-reactivity or interference between APOA5 and analogues was observed.
• Sequence Length: 367

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.077ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level APOA5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level APOA5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA5 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of APOA5 in rat serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APOA5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APOA5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APOA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APOA5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 471270246
• NCBI GeneID: 140638
• NCBI Accession #: NP_001264193.1
• NCBI GenBank Nucleotide #: NM_001277264.1
• UniProt Accession #: Q9QUH3; Q5FVT8
• Molecular Weight: 41,427 Da
• NCBI Official Full Name: apolipoprotein A-V
• NCBI Official Synonym Full Names: apolipoprotein A5
• NCBI Official Symbol: Apoa5
• NCBI Official Synonym Symbols: apo-AV; apoA-V
• NCBI Protein Information: apolipoprotein A-V
• UniProt Protein Name: Apolipoprotein A-V
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa5
• UniProt Synonym Gene Names: Rap3; Apo-AV; ApoA-V

NCBI Description

member of the apolipoprotein family; expression increases during liver regeneration [RGD, Feb 2006]

Uniprot Description

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages ().