Mouse anti-Human ANXA2 Monoclonal Antibody | anti-ANXA2 antibody

ANXA2 (Annexin A2, Annexin II, Annexin-2, Calpactin I Heavy Chain, Calpactin-1 Heavy Chain, Chromobindin-8, Lipocortin II, Placental Anticoagulant Protein IV, PAP-IV, Protein I, p36, ANX2, ANX2L4, CAL1H, LPC2D) (HRP)

Cat. Num. AAA6151217

• Gene Names: ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ANXA2; Monoclonal Antibody; ANXA2 (Annexin A2; Annexin II; Annexin-2; Calpactin I Heavy Chain; Calpactin-1 Heavy Chain; Chromobindin-8; Lipocortin II; Placental Anticoagulant Protein IV; PAP-IV; Protein I; p36; ANX2; ANX2L4; CAL1H; LPC2D) (HRP); anti-ANXA2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3E8-B6
• Specificity: Recognizes human ANXA2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-ANXA2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-339 from human ANXA2 (AAH09564) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MFTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETLILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (63.03kD).)

Western Blot (WB)

(ANXA2 monoclonal antibody Western Blot analysis of ANXA2 expression in Hela.)

Western Blot (WB)

(Western Blot analysis of ANXA2 expression in transfected 293T cell line by ANXA2 monoclonal antibody. Lane 1: ANXA2 transfected lysate (40.4kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ANXA2 on HeLa cell. [antibody concentration 10ug/ml].)

Western Blot (WB)

(Western blot analysis of ANXA2 over-expressed 293 cell line, cotransfected with ANXA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ANXA2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Related Product Information for anti-ANXA2 antibody

Annexin A2 (ANXA2), is an ~40kD member of the Annexin family of calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. Phosphorylation of Annexin A2 at Tyr23 regulates its involvement in endosomal trafficking and actin cytoskeleton rearrangement. The Annexins consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C-terminal domain is comprised of four 60-70aa annexin repeats. Annexin A2 also functions as an autocrine factor to enhance osteoclast formation and bone resorption and is a major cellular substrate of the tyrosine kinase Src. Human Annexin A2 shares 97% identity with mouse and rat Annexin A2.

Product Categories/Family for anti-ANXA2 antibody

Antibodies; Binding Proteins

References

1. Peripheral blood monocyte-expressed ANXA2 geneis involved in pathogenesis of osteoporosis in humans. Deng FY, Lei SF, Zhang Y, Zhang YL, Zheng YP, Zhang LS, Pan R, Wang L, Tian Q, Shen H, Zhao M, Lundberg YW, Liu YZ, Papasian CJ, Deng HW.Mol Cell Proteomics. 2011 Aug 4.

NCBI and Uniprot Product Information

• NCBI GI #: 16306977
• NCBI GeneID: 302
• UniProt Accession #: P07355; Q567R4; Q6N0B3; Q8TBV2; Q96DD5; Q9UDH8
• Molecular Weight: 38,604 Da
• NCBI Official Full Name: Homo sapiens annexin A2, mRNA
• NCBI Official Synonym Full Names: annexin A2
• NCBI Official Symbol: ANXA2
• NCBI Official Synonym Symbols: P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV
• NCBI Protein Information: annexin A2; annexin-2; protein I; annexin II; lipocortin II; chromobindin 8; chromobindin-8; calpactin I heavy chain; calpactin-1 heavy chain; calpactin I heavy polypeptide; placental anticoagulant protein IV
• UniProt Protein Name: Annexin A2
• Protein Family: Annexin
• UniProt Gene Name: ANXA2
• UniProt Synonym Gene Names: ANX2; ANX2L4; CAL1H; LPC2D; PAP-IV
• UniProt Entry Name: ANXA2_HUMAN

NCBI Description

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA2: a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. Heterotetramer containing 2 light chains of S100A10 2 heavy chains of ANXA2. May cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Motility/polarity/chemotaxis; Lipid-binding; Calcium-binding

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: extrinsic to plasma membrane; extracellular space; protein complex; cell surface; lysosomal membrane; late endosome membrane; early endosome; lipid particle; cell cortex; lipid raft; extracellular matrix; ruffle; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; basement membrane; midbody; nucleus; sarcolemma; endosome; vesicle

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; cytoskeletal protein binding; calcium ion binding; calcium-dependent protein binding; Rab GTPase binding

Biological Process: positive regulation of fibroblast proliferation; fibrinolysis; collagen fibril organization; negative regulation of catalytic activity; protein heterotetramerization; positive regulation of vesicle fusion; angiogenesis; positive regulation of binding; positive regulation of protein amino acid phosphorylation; lipid raft formation; body fluid secretion; membrane budding