Rabbit anti-Human GSTM2 Polyclonal Antibody | anti-GSTM2 antibody

Anti-GSTM2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106111

• Gene Names: GSTM2; GST4; GSTM; GTHMUS; GSTM2-2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: GSTM2; Polyclonal Antibody; Anti-GSTM2 Antibody; Rabbit Polyclonal; GSTM2 Antibody; Rabbit PAb; Antigen Affinity Purified; glutathione S-transferase mu 2 (muscle); Anti-GST4 Antibody; Anti-GSTM Antibody; Anti-GSTM2-2 Antibody; Anti-GTHMUS Antibody; anti-GSTM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human GSTM2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GSTM2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Human GSTM2 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human GSTM2 (rh GSTM2). GSTM2 specific IgG was purified by Human GSTM2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-GSTM2 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:26 kDa)

Related Product Information for anti-GSTM2 antibody

Glutathione S-transferase Mu 2, also known as GST class-mu 2, GSTM2-2, and GSTM2, is a cytoplasm protein that belongs to the GST superfamily and Mu family. GSTM2/GST4 contains one GST C-terminal domain and one GST N-terminal domain. The glutathione S-transferases (GSTs) are a multigene family of enzymes largely involved in the detoxification of chemicals. Eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta, and zeta. Butyrate, an important luminal component produced from the fermentation of dietary fibers, is an efficient inducer of GSTs and especially of GSTM2. Butyrate may act chemoprotective by increasing detoxification capabilities in the colon mucosa.

References

Campbell E, et al., 1990, J Biol Chem 265 (16): 9188-93. Vorachek WR, et al., 1991, Proc Natl Acad Sci USA. 88 (10): 4443-7. Ebert, M.N. et al., 2003, Carcinogenesis. 24 (10):1637-44.

NCBI and Uniprot Product Information

• NCBI GI #: 215277000
• NCBI GeneID: 2946
• NCBI Accession #: NP_001135840.1
• NCBI GenBank Nucleotide #: NM_001142368.1
• UniProt Accession #: P28161; Q2M318; Q5TZY5; Q8WWE1; B4DRY4; E9PEM9
• Molecular Weight: 25,745 Da
• NCBI Official Full Name: glutathione S-transferase Mu 2 isoform 2
• NCBI Official Synonym Full Names: glutathione S-transferase mu 2 (muscle)
• NCBI Official Symbol: GSTM2
• NCBI Official Synonym Symbols: GST4; GSTM; GTHMUS; GSTM2-2
• NCBI Protein Information: glutathione S-transferase Mu 2; GST, muscle; GST class-mu 2; glutathione S-transferase 4; glutathione S-transferase M1; glutathione S-aryltransferase M2; glutathione S-alkyltransferase M2; glutathione S-aralkyltransferase M2; S-(hydroxyalkyl)glutathione l
• UniProt Protein Name: Glutathione S-transferase Mu 2
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTM2
• UniProt Synonym Gene Names: GST4
• UniProt Entry Name: GSTM2_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTM2: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family.

Protein type: Transferase; Motility/polarity/chemotaxis; EC 2.5.1.18

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: sarcoplasmic reticulum; cytoplasm; cytosol

Molecular Function: protein homodimerization activity; enzyme binding; glutathione transferase activity; glutathione binding; receptor binding

Biological Process: regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; glutathione metabolic process; nitrobenzene metabolic process; xenobiotic metabolic process; xenobiotic catabolic process