Human Apolipoprotein A5 (APOA5) ELISA Kit | APOA5 elisa kit

Human Apolipoprotein A5 (APOA5) ELISA Kit

Cat. Num. AAA2704029

• Gene Names: APOA5; RAP3; APOAV
• Reactivity: Human
• Synonyms: Apolipoprotein A5 (APOA5); Human Apolipoprotein A5 (APOA5) ELISA Kit; Apo-A5; APOA-V; APOAV; RAP3; Regeneration-associated protein 3; APOA5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A5 (APOA5).; No significant cross-reactivity or interference between Apolipoprotein A5 (APOA5) and analogues was observed.
• Sequence Length: 366

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 1.25-80ng/mL
• Sensitivity: < 0.48ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A5 (APOA5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A5 (APOA5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A5 (APOA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A5 (APOA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A5 (APOA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein A5 (APOA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 262231737
• NCBI GeneID: 116519
• NCBI Accession #: NP_001160070.1
• NCBI GenBank Nucleotide #: NM_001166598.1
• UniProt Accession #: Q6Q788; Q3MIK6; Q6UWK9; Q9UBJ3; B0YIV9
• Molecular Weight: 41,213 Da
• NCBI Official Full Name: apolipoprotein A-V
• NCBI Official Synonym Full Names: apolipoprotein A5
• NCBI Official Symbol: APOA5
• NCBI Official Synonym Symbols: RAP3; APOAV
• NCBI Protein Information: apolipoprotein A-V
• UniProt Protein Name: Apolipoprotein A-V
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA5
• UniProt Synonym Gene Names: RAP3; Apo-AV; ApoA-V

NCBI Description

The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009]

Uniprot Description

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages.