Rat Alpha-Fetoprotein (aFP) ELISA Kit | aFP elisa kit

Rat Alpha-Fetoprotein (aFP) ELISA Kit

Cat. Num. AAA2701407

• Gene Names: AFP; AFPD; FETA; HPAFP
• Reactivity: Rat
• Synonyms: Alpha-Fetoprotein (aFP); Rat Alpha-Fetoprotein (aFP) ELISA Kit; A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein; aFP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Alpha-Fetoprotein (aFP).; No significant cross-reactivity or interference between Alpha-Fetoprotein (aFP) and analogues was observed.
• Sequence Length: 609

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.127ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Fetoprotein (aFP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Fetoprotein (aFP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for aFP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fetoprotein (aFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fetoprotein (aFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fetoprotein (aFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Fetoprotein (aFP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4501989
• NCBI GeneID: 174
• NCBI Accession #: NP_001125.1
• NCBI GenBank Nucleotide #: NM_001134.2
• UniProt Accession #: P02771; B2RBU3
• Molecular Weight: 68,678 Da
• NCBI Official Full Name: alpha-fetoprotein isoform 1
• NCBI Official Synonym Full Names: alpha fetoprotein
• NCBI Official Symbol: AFP
• NCBI Official Synonym Symbols: AFPD; FETA; HPAFP
• NCBI Protein Information: alpha-fetoprotein
• UniProt Protein Name: Alpha-fetoprotein
• Protein Family: Alpha-fetoprotein
• UniProt Gene Name: AFP
• UniProt Synonym Gene Names: HPAFP

NCBI Description

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Uniprot Description

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.