Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Synaptotagmin-7 (SYT7) Standard Curve.)
Mouse Synaptotagmin-7 (SYT7) ELISA Kit | SYT7 elisa kit
Mouse Synaptotagmin-7 (SYT7) ELISA Kit
Gene Names
Syt7; AI851541; B230112P13Rik
Reactivity
Mouse
Synonyms
Synaptotagmin-7 (SYT7); Mouse Synaptotagmin-7 (SYT7) ELISA Kit; SYT7; IPCA-7; MGC150517; PCANAP7; SYT-VII; prostate cancer associated protein 7; synaptotagmin 7; SYT7 elisa kit
Reactivity
Mouse
Specificity
Mouse Synaptotagmin-7 (SYT7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYT7. No significant cross-reactivity or interference between Mouse SYT7 and analogues was observed.
Sequence Length
403
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Synaptotagmin-7 (SYT7) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Synaptotagmin-7 (SYT7) Standard Curve.)
Related Product Information for SYT7 elisa kit
Description: This Mouse Synaptotagmin-7 (SYT7) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT7 in samples. An antibody specific for SYT7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYT7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SYT7 is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. A similar protein in rodents mediates hormone secretion and lysosome exocytosis. In humans, expression of SYT7 has been associated with prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for SYT7.
Background: SYT7 is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. A similar protein in rodents mediates hormone secretion and lysosome exocytosis. In humans, expression of SYT7 has been associated with prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for SYT7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
synaptotagmin-7 isoform alpha
NCBI Official Synonym Full Names
synaptotagmin VII
NCBI Official Symbol
Syt7
NCBI Official Synonym Symbols
AI851541; B230112P13Rik
NCBI Protein Information
synaptotagmin-7
UniProt Protein Name
Synaptotagmin-7
Protein Family
UniProt Gene Name
Syt7
UniProt Synonym Gene Names
SytVII
UniProt Entry Name
SYT7_MOUSE
Uniprot Description
SYT7: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Belongs to the synaptotagmin family.
Protein type: Membrane protein, integral
Cellular Component: membrane; lysosome; integral to membrane; plasma membrane; synapse; cytoplasmic vesicle; cell junction
Molecular Function: clathrin binding; syntaxin binding; calcium-dependent phospholipid binding; metal ion binding; calcium ion binding
Biological Process: vesicle fusion; synaptic vesicle exocytosis; exocytosis; plasma membrane repair; calcium ion-dependent exocytosis of neurotransmitter; regulation of calcium ion-dependent exocytosis
Research Articles on SYT7
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Product Notes
The Mouse SYT7 syt7 (Catalog #AAA9716917) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716917 ELISA Kit recognizes Mouse SYT7. It is sometimes possible for the material contained within the vial of "Synaptotagmin-7 (SYT7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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