Rat Agouti Related Protein, AGRP ELISA Kit | AGRP elisa kit

Rat Agouti Related Protein, AGRP ELISA Kit

Cat. Num. AAA705359

• Gene Names: AGRP; ART; AGRT; ASIP2
• Reactivity: Rat
• Synonyms: Agouti Related Protein; AGRP; Rat Agouti Related Protein; AGRP ELISA Kit; AGRP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat AGRP. No significant cross-reactivity or interference between rat AGRP and analogues was observed.
• Sequence Length: 132

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml -10 ng/ml.
• Sensitivity: The minimum detectable dose of rat AGRP is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGRP elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AGRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGRP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGRP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4501995
• NCBI GeneID: 181
• NCBI Accession #: NP_001129.1
• NCBI GenBank Nucleotide #: NM_001138.1
• UniProt Accession #: O00253; O15459; Q2TBD9
• Molecular Weight: 14,440 Da
• NCBI Official Full Name: agouti-related protein
• NCBI Official Synonym Full Names: agouti related neuropeptide
• NCBI Official Symbol: AGRP
• NCBI Official Synonym Symbols: ART; AGRT; ASIP2
• NCBI Protein Information: agouti-related protein; agouti related protein homolog
• UniProt Protein Name: Agouti-related protein
• Protein Family: Agouti-related protein
• UniProt Gene Name: AGRP
• UniProt Synonym Gene Names: AGRT; ART
• UniProt Entry Name: AGRP_HUMAN

NCBI Description

This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009]

Uniprot Description

Function: Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner.5 PublicationsManual assertion based on experiment in:Ref.8