Sheep Agouti Related Protein ELISA Kit | AGRP elisa kit

Sheep Agouti Related Protein ELISA Kit

Cat. Num. AAA743767

• Gene Names: AGRP; ART; AGRT; ASIP2
• Reactivity: Sheep
• Synonyms: Agouti Related Protein; Sheep Agouti Related Protein ELISA Kit; AGRP elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This AGRP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep AGRP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGRP elisa kit

Principle of the Assay: AGRP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGRP antibody and an AGRP-HRP conjugate. The assay sample and buffer are incubated together with AGRP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGRP concentration since AGRP from samples and AGRP-HRP conjugate compete for the anti-AGRP antibody binding site. Since the number of sites is limited, as more sites are occupied by AGRP from the sample, fewer sites are left to bind AGRP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGRP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AGRP elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4501995
• NCBI GeneID: 181
• NCBI Accession #: NP_001129.1
• NCBI GenBank Nucleotide #: NM_001138.1
• Molecular Weight: 14,440 Da
• NCBI Official Full Name: agouti-related protein
• NCBI Official Synonym Full Names: agouti related neuropeptide
• NCBI Official Symbol: AGRP
• NCBI Official Synonym Symbols: ART; AGRT; ASIP2
• NCBI Protein Information: agouti-related protein; agouti related protein homolog
• UniProt Protein Name: Agouti-related protein
• Protein Family: Agouti-related protein
• UniProt Gene Name: AGRP
• UniProt Synonym Gene Names: AGRT; ART
• UniProt Entry Name: AGRP_HUMAN

NCBI Description

This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009]

Uniprot Description

AGRP: Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner. Genetic variations in AGRP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.

Protein type: Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: extracellular space; cell soma; Golgi lumen

Molecular Function: neuropeptide hormone activity; receptor binding

Biological Process: maternal process involved in pregnancy; hormone-mediated signaling; neuropeptide signaling pathway; eating behavior; photoperiodism; feeding behavior; adult feeding behavior; response to insulin stimulus

Disease: Obesity