Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pituitary adenylate cyclase activating polypeptide (PACAP) Standard Curve.)
Human Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) ELISA Kit | PACAP elisa kit
Human Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pituitary adenylate cyclase activating polypeptide (PACAP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pituitary adenylate cyclase activating polypeptide (PACAP) Standard Curve.)
Background: Pituitary adenylate cyclase-activating peptide, adenylate cyclase-activating polypeptide 1. Mediated by adenylate cyclase-activating polypeptide 1 receptors, this polypeptide stimulates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase-activating polypeptide 1 not only is a hypophysiotropic hormone but also functions as a neurotransmitter and neuromodulator. In addition, it plays a role in paracrine and autocrine regulation of certain types of cells. This gene is composed of five exons. Exons 1 and 2 encode the 5' UTR and signal peptide, respectively; exon 4 encodes an adenylate cyclase-activating polypeptide 1-related peptide; and exon 5 encodes the mature peptide and 3' UTR.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]
Uniprot Description
ADCYAP1: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Belongs to the glucagon family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 18p11
Cellular Component: extracellular space; extracellular region; terminal button
Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; receptor signaling protein activity; pituitary adenylate cyclase-activating peptide receptor binding; pituitary adenylate cyclase activating polypeptide activity; receptor binding
Biological Process: behavioral fear response; nerve growth factor receptor signaling pathway; negative regulation of potassium ion transport; female pregnancy; sensory perception of pain; positive regulation of vasodilation; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell cycle; histamine secretion; elevation of cytosolic calcium ion concentration; cAMP-mediated signaling; regulation of protein localization; cell-cell signaling; ovarian follicle development; neuropeptide signaling pathway; positive regulation of cell proliferation; neurite development; negative regulation of acute inflammatory response to antigenic stimulus; adenylate cyclase activation; positive regulation of interleukin-6 production; response to starvation; positive regulation of growth hormone secretion; ATP metabolic process; response to ethanol; regulation of postsynaptic membrane potential; pituitary gland development; positive regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of acute inflammatory response to non-antigenic stimulus; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of G-protein coupled receptor protein signaling pathway
Research Articles on PACAP
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Product Notes
The Human PACAP adcyap1 (Catalog #AAA9714598) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714598 ELISA Kit recognizes Human PACAP. It is sometimes possible for the material contained within the vial of "Pituitary Adenylate Cyclase Activating Polypeptide (PACAP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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