Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Adenosine receptor A1 (ADORA1) Standard Curve.)
Mouse Adenosine Receptor A1 (ADORA1) ELISA Kit | ADORA1 elisa kit
Mouse Adenosine Receptor A1 (ADORA1) ELISA Kit
Gene Names
Adora1; Ri; A1R; A1AR; AA1R; ARA1; A1-AR; AI848715; BB176431
Reactivity
Mouse
Synonyms
Adenosine Receptor A1 (ADORA1); Mouse Adenosine Receptor A1 (ADORA1) ELISA Kit; ADORA1; RDC7; OTTHUMP00000039091; ADORA1 elisa kit
Reactivity
Mouse
Specificity
Mouse Adenosine receptor A1 (ADORA1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ADORA1. No significant cross-reactivity or interference between Mouse ADORA1 and analogues was observed.
Sequence Length
326
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Adenosine receptor A1 (ADORA1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Adenosine receptor A1 (ADORA1) Standard Curve.)
Related Product Information for ADORA1 elisa kit
Description: This Mouse Adenosine receptor A1 (ADORA1) ELISA Kit employs a two-site sandwich ELISA to quantitate ADORA1 in samples. An antibody specific for ADORA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADORA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADORA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADORA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The receptor is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. Activation of the adenosine A1 receptor by an agonist causes binding of Gi1/2/3 or Go protein. Binding of Gi1/2/3 causes an inhibition of adenylate cyclase and therefore a decrease in the cAMP concentration.
Background: The receptor is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. Activation of the adenosine A1 receptor by an agonist causes binding of Gi1/2/3 or Go protein. Binding of Gi1/2/3 causes an inhibition of adenylate cyclase and therefore a decrease in the cAMP concentration.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
adenosine receptor A1 isoform a
NCBI Official Synonym Full Names
adenosine A1 receptor
NCBI Official Symbol
Adora1
NCBI Official Synonym Symbols
Ri; A1R; A1AR; AA1R; ARA1; A1-AR; AI848715; BB176431
NCBI Protein Information
adenosine receptor A1
UniProt Protein Name
Adenosine receptor A1
Protein Family
UniProt Gene Name
Adora1
Uniprot Description
Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Research Articles on ADORA1
Similar Products
Product Notes
The Mouse ADORA1 adora1 (Catalog #AAA9717169) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717169 ELISA Kit recognizes Mouse ADORA1. It is sometimes possible for the material contained within the vial of "Adenosine Receptor A1 (ADORA1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
