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Western Blot (WB) (Western Blot: Sample: Recombinant BMP2, Rat.)

Rabbit anti-Rat Bone Morphogenetic Protein 2 (BMP2) Polyclonal Antibody | anti-BMP2 antibody

Polyclonal Antibody to Bone Morphogenetic Protein 2 (BMP2)

Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Bone Morphogenetic Protein 2 (BMP2); Polyclonal Antibody; Polyclonal Antibody to Bone Morphogenetic Protein 2 (BMP2); anti-BMP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against BMP2. It has been selected for its ability to recognize BMP2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.32mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- SE FELRLLSMFG LKQRPTPSKD VVVPPYMLDL YRRHSGQPGA LAPDHRLERA ASRANTVLSF HHEEAIEELS EMSGKTSRRF FFNLSSVPTD EFLTSAELQI FREQMQEALG NSSFQHRINI YEIIKPATAS SKFPVTRLLD TRLVTQNTSQ WESFDVTPAV MRWTAQGHTN HGFVVEVAHL EEKPGVSKRH VRI
Sequence Length
396
Applicable Applications for anti-BMP2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant BMP2 (Ser49~Ile243) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2035153
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant BMP2, Rat.)

Western Blot (WB) (Western Blot: Sample: Recombinant BMP2, Rat.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:RatTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:RatTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,383 Da
NCBI Official Full Name
bone morphogenetic protein 2
NCBI Official Synonym Full Names
bone morphogenetic protein 2
NCBI Official Symbol
Bmp2
NCBI Protein Information
bone morphogenetic protein 2
UniProt Protein Name
Bone morphogenetic protein 2
UniProt Gene Name
Bmp2
UniProt Synonym Gene Names
Bmp-2; BMP-2; BMP-2A

NCBI Description

involved in cellular signaling during limb development; induces bone formation [RGD, Feb 2006]

Uniprot Description

BMP2: Induces cartilage and bone formation. Belongs to the TGF-beta family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q36

Cellular Component: cell surface; cytoplasm; extracellular space; intracellular membrane-bound organelle; protein complex; vesicle

Molecular Function: cytokine activity; growth factor activity; phosphatase activator activity; protein domain specific binding; protein heterodimerization activity; protein homodimerization activity; receptor binding; retinol dehydrogenase activity; SMAD binding; transforming growth factor beta receptor binding

Biological Process: activation of MAPK activity; BMP signaling pathway; bone mineralization; branching involved in ureteric bud morphogenesis; cardiac cell differentiation; cardiac muscle cell differentiation; cardiac muscle morphogensis; cartilage development; cell fate commitment; chondrocyte differentiation; corticotropin hormone secreting cell differentiation; embryonic heart tube anterior/posterior pattern formation; epithelial to mesenchymal transition; heart development; in utero embryonic development; inflammatory response; inner ear development; mesenchymal cell differentiation; negative regulation of aldosterone biosynthetic process; negative regulation of calcium-independent cell-cell adhesion; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of insulin-like growth factor receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; Notch signaling pathway; odontogenesis of dentine-containing teeth; organ morphogenesis; ossification; osteoblast differentiation; ovulation cycle; pericardium development; positive regulation of apoptosis; positive regulation of astrocyte differentiation; positive regulation of bone mineralization; positive regulation of cell differentiation; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of fat cell differentiation; positive regulation of MAPK cascade; positive regulation of neurogenesis; positive regulation of neuron differentiation; positive regulation of odontogenesis; positive regulation of ossification; positive regulation of osteoblast differentiation; positive regulation of osteoblast proliferation; positive regulation of protein binding; positive regulation of protein phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of Wnt receptor signaling pathway; protein amino acid phosphorylation; protein destabilization; proteoglycan metabolic process; regulation of apoptosis; regulation of MAPK cascade; regulation of odontogenesis of dentine-containing teeth; regulation of transcription, DNA-templated; response to hypoxia; response to mechanical stimulus; response to retinoic acid; telencephalon development; telencephalon regionalization; thyroid-stimulating hormone-secreting cell differentiation

Research Articles on BMP2

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Product Notes

The BMP2 bmp2 (Catalog #AAA2003856) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Bone Morphogenetic Protein 2 (BMP2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Bone Morphogenetic Protein 2 (BMP2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the BMP2 bmp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- SE FELRLLSMFG LKQRPTPSKD VVVPPYMLDL YRRHSGQPGA LAPDHRLERA ASRANTVLSF HHEEAIEELS EMSGKTSRRF FFNLSSVPTD EFLTSAELQI FREQMQEALG NSSFQHRINI YEIIKPATAS SKFPVTRLLD TRLVTQNTSQ WESFDVTPAV MRWTAQGHTN HGFVVEVAHL EEKPGVSKRH VRI. It is sometimes possible for the material contained within the vial of "Bone Morphogenetic Protein 2 (BMP2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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