Rat Adenosine receptor A1 (ADORA1) ELISA Kit | ADORA1 elisa kit

Rat Adenosine receptor A1 (ADORA1) ELISA Kit

Cat. Num. AAA7213827

• Gene Names: ADORA1; RDC7
• Reactivity: Rat
• Synonyms: Adenosine receptor A1 (ADORA1); Rat Adenosine receptor A1 (ADORA1) ELISA Kit; ADORA1 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This ADORA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ADORA1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADORA1 elisa kit

Principle of the assay: ADORA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADORA1 antibody and an ADORA1-HRP conjugate. The assay sample and buffer are incubated together with ADORA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADORA1 concentration since ADORA1 from samples and ADORA1-HRP conjugate compete for the anti-ADORA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADORA1 from the sample, fewer sites are left to bind ADORA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADORA1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADORA1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4501947
• NCBI GeneID: 134
• NCBI Accession #: NP_000665.1
• NCBI GenBank Nucleotide #: NM_000674.2
• Molecular Weight: 13,348 Da
• NCBI Official Full Name: adenosine receptor A1
• NCBI Official Synonym Full Names: adenosine A1 receptor
• NCBI Official Symbol: ADORA1
• NCBI Official Synonym Symbols: RDC7
• NCBI Protein Information: adenosine receptor A1; adenosine A1 receptor variant 1
• UniProt Protein Name: Adenosine receptor A1
• Protein Family: Adenosine receptor
• UniProt Gene Name: ADORA1
• UniProt Entry Name: AA1R_HUMAN

NCBI Description

The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ADORA1: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Apoptosis; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: basolateral plasma membrane; integral to plasma membrane; endoplasmic reticulum; postsynaptic density; dendritic spine; terminal button; axolemma; asymmetric synapse; presynaptic membrane; postsynaptic membrane; cell soma; plasma membrane; presynaptic active zone

Molecular Function: protein binding; G-protein-coupled receptor binding; protein heterodimerization activity; G-protein beta/gamma-subunit binding; purine nucleoside binding; adenosine receptor activity, G-protein coupled; phospholipase C activity; heterotrimeric G-protein binding

Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; relaxation of vascular smooth muscle; activation of MAPKK activity; negative regulation of glutamate secretion; negative regulation of hormone secretion; positive regulation of potassium ion transport; regulation of synaptic plasticity; positive regulation of peptide secretion; neurological control of breathing; thermoregulation; signal transduction; positive regulation of protein amino acid dephosphorylation; negative regulation of cell proliferation; regulation of glomerular filtration; negative regulation of vasodilation; cell-cell signaling; positive regulation of epidermal growth factor receptor activity; positive regulation of nucleoside transport; negative regulation of lipid catabolic process; negative regulation of blood pressure; negative regulation of synaptic transmission, GABAergic; inflammatory response; detection of temperature stimulus involved in sensory perception of pain; protein targeting to membrane; adenosine receptor signaling pathway; nervous system development; negative regulation of neurotrophin production; negative regulation of acute inflammatory response; negative regulation of synaptic transmission, glutamatergic; negative regulation of leukocyte migration; phagocytosis; response to hypoxia; G-protein signaling, adenylate cyclase inhibiting pathway; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; cognition; positive regulation of blood pressure; lipid catabolic process; negative regulation of apoptosis