SR-BI/SCARB1 Recombinant Protein | SCARB1 recombinant protein

SR-BI/SCARB1 Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8120666

• Gene Names: SCARB1; CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
• Purity: >90% as determined by SDS-PAGE
• Synonyms: SR-BI/SCARB1; SR-BI/SCARB1 Protein; Human; Recombinant (ECD; hFc Tag); Human SR-BI/SCARB1 Protein (Fc Tag); CD36L1 Protein; CLA-1 Protein; CLA1 Protein; HDLQTL6 Protein; SR-BI Protein; SRB1 Protein; scavenger receptor class B; member 1; SCARB1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Pro33-Tyr443

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Pro 33
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human SCARB1 (NP_001076428.1)(Pro33-Tyr443) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SCARB1 recombinant protein

Background: Scavenger receptor class B, member 1 (SCARB1), also known as CD36L1, is a member of the scavenger receptor family. SCARB1 is expressed primarily in liver and non placental steroidogenic tissues, and predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane. SCARB1 is proposed as a receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells, and is involved in a wide variety of physilogical processes. As a key component in the reverse cholesterol transport pathway, SCARB1 binds high density lipoproteins (HDLs) and mediates selective cholesterol uptake by a mechanism distinct from the LDL pathway. High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SCARB1 may thus serve as a useful marker that predicts variation in baseline lipid levels and postprandial lipid response. The mouse SCARB1 has been shown to exert actions in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.

References

Murao, K. et al., 1997, J. Biol. Chem. 272(28): 17551-17557. Ikemoto, M. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97 (12): 6538-6543. Husemann, J. et al., 2001, Am. J. Pathol. 158 (3): 825-832. Williams, D.L. et al., 2001, Endocr. Res. 26 (4): 639-651. Bulte, B. S. et al., 2002, J. Biol. Chem. 277 (39): 36092-36099. Duncan, K.G. et al., 2002, Biochem. Biophys. Res. Commun. 292 (4): 1017-1022.

NCBI and Uniprot Product Information

• NCBI GI #: 132566680
• NCBI GeneID: 949
• NCBI Accession #: NP_001076428.1
• NCBI GenBank Nucleotide #: NM_001082959.1
• UniProt Accession #: Q8WTV0; Q14016; Q52LZ5; Q6KFX4; F8W8N0
• Molecular Weight: 60878 MW
• NCBI Official Full Name: scavenger receptor class B member 1 isoform 2
• NCBI Official Synonym Full Names: scavenger receptor class B member 1
• NCBI Official Symbol: SCARB1
• NCBI Official Synonym Symbols: CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
• NCBI Protein Information: scavenger receptor class B member 1
• UniProt Protein Name: Scavenger receptor class B member 1
• Protein Family: Scavenger receptor
• UniProt Gene Name: SCARB1
• UniProt Synonym Gene Names: CD36L1; CLA1; SRB1; CLA-1
• UniProt Entry Name: SCRB1_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane receptor for high density lipoprotein cholesterol (HDL). The encoded protein mediates cholesterol transfer to and from HDL. In addition, this protein is a receptor for hepatitis C virus glycoprotein E2. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

SCARB1: Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester. Belongs to the CD36 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cell surface; lysosomal membrane; microvillus membrane; integral to plasma membrane; plasma membrane; caveola

Molecular Function: apolipoprotein A-I binding; protein binding; protein homodimerization activity; lipopolysaccharide binding; transporter activity; phosphatidylserine binding; low-density lipoprotein binding; lipopolysaccharide receptor activity; apolipoprotein binding; high-density lipoprotein binding; phosphatidylinositol binding

Biological Process: receptor-mediated endocytosis; wound healing; blood vessel endothelial cell migration; viral reproduction; positive regulation of nitric-oxide synthase activity; lipoprotein metabolic process; cholesterol efflux; androgen biosynthetic process; regulation of phagocytosis; recognition of apoptotic cell; lipopolysaccharide transport; cholesterol homeostasis; reverse cholesterol transport; detection of lipopolysaccharide; endothelial cell proliferation; lipopolysaccharide-mediated signaling pathway; adhesion to host; phospholipid transport; cholesterol catabolic process; cell adhesion

Disease: High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 6