Immunohistochemistry (IHC)
(Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Rabbit PPARG / PPAR Gamma Polyclonal Antibody | anti-PPARG antibody
Rabbit Polyclonal to Human PPARG / PPAR Gamma
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
Immunohistochemistry (IHC)
(Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(Anti-PPAR2 Antibody - Western Blot. Western blot of affinity purified anti-PPAR2 antibody shows detection of PPAR2 protein in a mouse 3T3 whole cell lysate (lane 1 arrowhead). Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose. Primary antibody was used at a 1:2000 dilution in 5% BLOTTO PBS solution. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.)
Western Blot (WB)
(Anti-PPAR2 Antibody - Western Blot. Western blot of affinity purified anti-PPAR2 antibody shows detection of PPAR2 protein in a mouse 3T3 whole cell lysate (lane 1 arrowhead). Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose. Primary antibody was used at a 1:2000 dilution in 5% BLOTTO PBS solution. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 3p25
Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytosol; nucleus
Molecular Function: ligand-dependent nuclear receptor activity; transcription activator binding; zinc ion binding; drug binding; alpha-actinin binding; protein phosphatase binding; retinoid X receptor binding; arachidonic acid binding; protein binding; enzyme binding; DNA binding; ligand-dependent nuclear receptor transcription coactivator activity; prostaglandin receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; estrogen receptor binding; chromatin binding; transcription factor activity
Biological Process: negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; heart development; cell maturation; rhythmic process; lipid homeostasis; response to lipid; glucose homeostasis; response to caffeine; response to vitamin A; positive regulation of oligodendrocyte differentiation; placenta development; long-chain fatty acid transport; organ regeneration; cell fate commitment; monocyte differentiation; negative regulation of acute inflammatory response; regulation of circadian rhythm; response to starvation; negative regulation of telomerase activity; cellular response to insulin stimulus; response to mechanical stimulus; lipoprotein transport; response to estrogen stimulus; brown fat cell differentiation; positive regulation of fat cell differentiation; fatty acid oxidation; positive regulation of transcription factor activity; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of phagocytosis, engulfment; negative regulation of smooth muscle cell proliferation; low-density lipoprotein receptor biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; epithelial cell differentiation; regulation of blood pressure; response to nutrient; caspase activation; transcription initiation from RNA polymerase II promoter; response to retinoic acid; G-protein coupled receptor protein signaling pathway; response to low density lipoprotein stimulus; white fat cell differentiation; positive regulation of fatty acid oxidation; innate immune response; gene expression; response to cold; negative regulation of cell growth; lipid metabolic process
Disease: Obesity; Carotid Intimal Medial Thickness 1; Lipodystrophy, Familial Partial, Type 3; Diabetes Mellitus, Noninsulin-dependent
Research Articles on PPARG
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Product Notes
The PPARG pparg (Catalog #AAA240836) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human PPARG / PPAR Gamma reacts with Human, Macaque and may cross-react with other species as described in the data sheet. AAA Biotech's PPARG / PPAR Gamma can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:3200 - 1:10000), IHC-P (2.5 ug/ml), WB (1:1000 - 1:3000) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.. Researchers should empirically determine the suitability of the PPARG pparg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPARG / PPAR Gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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