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Typical Testing Data/Standard Curve (for reference only)

Rat ADAMTS13/VWFCP ELISA Kit | ADAMTS13/VWFCP elisa kit

Rat ADAMTS13/VWFCP(Von Willebrand Factor Cleaving Protease)ELISA Kit

Gene Names
ADAMTS13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
Reactivity
Rat
Synonyms
ADAMTS13/VWFCP; Rat ADAMTS13/VWFCP(Von Willebrand Factor Cleaving Protease)ELISA Kit; ADAMTS13/VWFCP elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This kit recognizes Rat ADAMTS13/VWFCP in samples. No significant cross-reactivity or interference between Rat ADAMTS13/VWFCP and analogues was observed.
Sequence Length
1371
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
78.13-5000pg/mL
Sensitivity
46.88pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ADAMTS13/VWFCP were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ADAMTS13/VWFCP were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ADAMTS13/VWFCP elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat ADAMTS13/VWFCP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ADAMTS13/VWFCP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ADAMTS13/VWFCP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ADAMTS13/VWFCP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat ADAMTS13/VWFCP. You can calculate the concentration of Rat ADAMTS13/VWFCP in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,864 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 2 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif, 13
NCBI Official Symbol
ADAMTS13
NCBI Official Synonym Symbols
VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13; vWF-cleaving protease; von Willebrand factor-cleaving protease
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 13
UniProt Gene Name
ADAMTS13
UniProt Synonym Gene Names
C9orf8; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease
UniProt Entry Name
ATS13_HUMAN

NCBI Description

This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.87; Extracellular matrix; Motility/polarity/chemotaxis; Secreted, signal peptide; Calcium-binding; Protease; Secreted

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; endoplasmic reticulum lumen

Molecular Function: integrin binding; protein binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding

Biological Process: integrin-mediated signaling pathway; protein amino acid O-linked glycosylation; platelet activation; cellular protein metabolic process; glycoprotein metabolic process; cell-matrix adhesion; peptide catabolic process; response to toxin; protein processing; post-translational protein modification; proteolysis

Disease: Thrombotic Thrombocytopenic Purpura, Congenital

Research Articles on ADAMTS13/VWFCP

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Product Notes

The Rat ADAMTS13/VWFCP adamts13 (Catalog #AAA2509576) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2509576 ELISA Kit recognizes Rat ADAMTS13/VWFCP. It is sometimes possible for the material contained within the vial of "ADAMTS13/VWFCP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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