Human A disintegrin and metalloproteinase with thrombospondin motifs 13 ELISA Kit | ADAMTS13 elisa kit

Human A disintegrin and metalloproteinase with thrombospondin motifs 13 ELISA Kit

Cat. Num. AAA766026

• Gene Names: ADAMTS13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
• Reactivity: Human
• Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 13; Human A disintegrin and metalloproteinase with thrombospondin motifs 13 ELISA Kit; ADAMTS13/VWFCP/TTP/vWF-CP/a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif; 13/ADAM metallopeptidase with thrombospondin type 1 motif; 13/ADAM-TS13/ADAMTS-13/vWF-cleaving protease; ADAMTS13 elisa kit

• Reactivity: Human
• Sequence Length: 1427

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ADAMTS13 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human ADAMTS13antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human ADAMTS13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAMTS13 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman ADAMTS13can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 21265034
• NCBI GeneID: 11093
• NCBI Accession #: NP_620594.1
• NCBI GenBank Nucleotide #: NM_139025.4
• Molecular Weight: 39,864 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 1 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif 13
• NCBI Official Symbol: ADAMTS13
• NCBI Official Synonym Symbols: VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 13
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 13
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS13
• UniProt Synonym Gene Names: C9orf8; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease
• UniProt Entry Name: ATS13_HUMAN

NCBI Description

This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding; Protease; Motility/polarity/chemotaxis; Secreted, signal peptide; Extracellular matrix; EC 3.4.24.87; Secreted

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: cell surface; endoplasmic reticulum lumen; extracellular space; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; integrin binding; metalloendopeptidase activity; metallopeptidase activity; protein binding; zinc ion binding

Biological Process: cell-matrix adhesion; cellular protein metabolic process; glycoprotein metabolic process; integrin-mediated signaling pathway; peptide catabolic process; platelet activation; post-translational protein modification; protein amino acid O-linked glycosylation; protein processing; proteolysis; response to toxin

Disease: Thrombotic Thrombocytopenic Purpura, Congenital