Rat ABCA13 (ATP Binding Cassette Transporter A13) ELISA Kit | ABCA13 elisa kit

Rat ABCA13 (ATP Binding Cassette Transporter A13) ELISA Kit

Cat. Num. AAA7606607

• Reactivity: Rat
• Synonyms: ABCA13 (ATP Binding Cassette Transporter A13); Rat ABCA13 (ATP Binding Cassette Transporter A13) ELISA Kit; ABCA13 elisa kit

• Reactivity: Rat
• Sequence Length: 5058

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 46.9-3000pg/ml
• Sensitivity: 28.1pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCA13 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ABCA13 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ABCA13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ABCA13 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ABCA13 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 597709765
• NCBI GeneID: 154664
• NCBI Accession #: NP_689914.3
• NCBI GenBank Nucleotide #: NM_152701.4
• UniProt Accession #: Q86UQ4; Q6ZTT7; Q86WI2; Q8N248; K9LC76; K9LC79; K9LCX7; K9LDK8; K9LDY4
• Molecular Weight: 258,321 Da
• NCBI Official Full Name: ATP-binding cassette sub-family A member 13
• NCBI Official Synonym Full Names: ATP binding cassette subfamily A member 13
• NCBI Official Symbol: ABCA13
• NCBI Protein Information: ATP-binding cassette sub-family A member 13
• UniProt Protein Name: ATP-binding cassette sub-family A member 13
• Protein Family: ATP-binding cassette sub-family
• UniProt Gene Name: ABCA13

NCBI Description

In human, the ATP-binding cassette (ABC) family of transmembrane transporters has at least 48 genes and 7 gene subfamilies. This gene is a member of ABC gene subfamily A (ABCA). Genes within the ABCA family typically encode several thousand amino acids. Like other ABC transmembrane transporter proteins, this protein has 12 or more transmembrane alpha-helix domains that likely arrange to form a single central chamber with multiple substrate binding sites. It is also predicted to have two large extracellular domains and two nucleotide binding domains as is typical for ABCA proteins. Alternative splice variants have been described but their biological validity has not been demonstrated.[provided by RefSeq, Mar 2009]

Uniprot Description

ABCA13: In human, the ATP-binding cassette (ABC) family of transmembrane transporters has at least 48 genes and 7 gene subfamilies. This gene is a member of ABC gene subfamily A (ABCA). Genes within the ABCA family typically encode several thousand amino acids. Like other ABC transmembrane transporter proteins, this protein has 12 or more transmembrane alpha-helix domains that likely arrange to form a single central chamber with multiple substrate binding sites. It is also predicted to have two large extracellular domains and two nucleotide binding domains as is typical for ABCA proteins. Alternative splice variants have been described but their biological validity has not been demonstrated.[provided by RefSeq, Mar 2009]

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: intracellular membrane-bound organelle; plasma membrane; secretory granule membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances

Biological Process: lipid transport; neutrophil degranulation